ALDH1A3 Rabbit Polyclonal Antibody
Catalog# HA500541
ALDH1A3 Rabbit Polyclonal Antibody
-
WB
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
ALDH1A3 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human ALDH1A3 aa 1-512 / 512.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 56 kDa
Positive Control
HepG2 cell lysate, SW1990 cell lysate, U-87 MG cell lysate, A431 cell lysate, human kidney tissue lysate, human liver tissue lysate, mouse kidney tissue lysate, rat liver tissue lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Aldehyde dehydrogenase 1 family, member A3, also known as ALDH1A3 or retinaldehyde dehydrogenase 3 (RALDH3), is an enzyme that in humans is encoded by the ALDH1A3 gene. Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or a cellular retinol-binding protein form. ALDH1A3 gene has been observed progressively downregulated in Human papillomavirus-positive neoplastic keratinocytes derived from uterine cervical preneoplastic lesions at different levels of malignancy. For this reason, ALDH1A3 is likely to be associated with tumorigenesis and may be a potential prognostic marker for uterine cervical preneoplastic lesions progression.
Background References
1. Li D et al. ALDH1A3 Coordinates Metabolism With Gene Regulation in Pulmonary Arterial Hypertension. Circulation. 2021 May
2. Son J et al. Genetic and pharmacologic inhibition of ALDH1A3 as a treatment of β-cell failure. Nat Commun. 2023 Feb
Subcellular Location
Cytoplasm.
Synonyms
Acetaldehyde dehydrogenase 6 antibody
AL1A3_HUMAN antibody
Aldehyde dehydrogenase 1A3 antibody
Aldehyde dehydrogenase 6 antibody
Aldehyde dehydrogenase family 1 member A3 antibody
Aldh1a3 antibody
ALDH1A6 antibody
ALDH6 antibody
RALDH 3 antibody
Retinaldehyde dehydrogenase 3 antibody
ExpandImages
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Western blot analysis of ALDH1A3 on different lysates with Rabbit anti-ALDH1A3 antibody (HA500541) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: SW1990 cell lysate (20 µg/Lane)
Lane 3: U-87 MG cell lysate (20 µg/Lane)
Lane 4: A431 cell lysate (20 µg/Lane)
Lane 5: Human kidney tissue lysate (40 µg/Lane)
Lane 6: Human liver tissue lysate (40 µg/Lane)
Lane 7: Mouse kidney tissue lysate (40 µg/Lane)
Lane 8: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500541) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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