ALDH1A3 Rabbit Polyclonal Antibody
Catalog# HA500541
ALDH1A3 Rabbit Polyclonal Antibody
-
WB
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
ALDH1A3 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human ALDH1A3 aa 1-512 / 512.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 56 kDa
Positive Control
HepG2 cell lysate, SW1990 cell lysate, U-87 MG cell lysate, A431 cell lysate, human kidney tissue lysate, human liver tissue lysate, mouse kidney tissue lysate, rat liver tissue lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Aldehyde dehydrogenase 1 family, member A3, also known as ALDH1A3 or retinaldehyde dehydrogenase 3 (RALDH3), is an enzyme that in humans is encoded by the ALDH1A3 gene. Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or a cellular retinol-binding protein form. ALDH1A3 gene has been observed progressively downregulated in Human papillomavirus-positive neoplastic keratinocytes derived from uterine cervical preneoplastic lesions at different levels of malignancy. For this reason, ALDH1A3 is likely to be associated with tumorigenesis and may be a potential prognostic marker for uterine cervical preneoplastic lesions progression.
Background References
1. Li D et al. ALDH1A3 Coordinates Metabolism With Gene Regulation in Pulmonary Arterial Hypertension. Circulation. 2021 May
2. Son J et al. Genetic and pharmacologic inhibition of ALDH1A3 as a treatment of β-cell failure. Nat Commun. 2023 Feb
Subcellular Location
Cytoplasm.
Synonyms
Acetaldehyde dehydrogenase 6 antibody
AL1A3_HUMAN antibody
Aldehyde dehydrogenase 1A3 antibody
Aldehyde dehydrogenase 6 antibody
Aldehyde dehydrogenase family 1 member A3 antibody
Aldh1a3 antibody
ALDH1A6 antibody
ALDH6 antibody
RALDH 3 antibody
Retinaldehyde dehydrogenase 3 antibody
ExpandImages
-
Western blot analysis of ALDH1A3 on different lysates with Rabbit anti-ALDH1A3 antibody (HA500541) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: SW1990 cell lysate (20 µg/Lane)
Lane 3: U-87 MG cell lysate (20 µg/Lane)
Lane 4: A431 cell lysate (20 µg/Lane)
Lane 5: Human kidney tissue lysate (40 µg/Lane)
Lane 6: Human liver tissue lysate (40 µg/Lane)
Lane 7: Mouse kidney tissue lysate (40 µg/Lane)
Lane 8: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500541) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
