ST3GAL5 Rabbit Polyclonal Antibody
Catalog# HA500547
ST3GAL5 Rabbit Polyclonal Antibody
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WB
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Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
ST3GAL5 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human ST3GAL5 aa 51-418.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 48 kDa
Positive Control
HEK-293 cell lysate, HeLa cell lysate, Jurkat cell lysate, U-87 MG cell lysate, Mouse lung tissue lysate, Mouse skeletal muscle tissue lysate, Rat lung tissue lysate, Rat skeletal muscle tissue lysate.
Conjugation
unconjugated
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.1% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:2,000
Target
Function
Transfers the sialyl group (N-acetyl-alpha-neuraminyl or NeuAc) from CMP-NeuAc to the non-reducing terminal galactose (Gal) of glycosphingolipids forming gangliosides (important molecules involved in the regulation of multiple cellular processes, including cell proliferation and differentiation, apoptosis, embryogenesis, development, and oncogenesis) . Mainly involved in the biosynthesis of ganglioside GM3 but can also use different glycolipids as substrate acceptors such as D-galactosylceramide (GalCer), asialo-GM2 (GA2) and asialo-GM1 (GA1), although less preferentially than beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)- Cer (LacCer) .
Background References
1. Dookwah M et al. Neural-specific alterations in glycosphingolipid biosynthesis and cell signaling associated with two human ganglioside GM3 synthase deficiency variants. Hum Mol Genet. 2023 Dec.
2. Watanabe S et al. Neurological insights on two siblings with GM3 synthase deficiency due to novel compound heterozygous ST3GAL5 variants. Brain Dev. 2023 May.
Subcellular Location
Golgi apparatus membrane Curated; Single-pass type II membrane protein
Synonyms
3-sialyltransferase
CMP-NeuAc:lactosylceramide alpha-2
CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransferase
EC 2.4.99.9
Ganglioside GM3 synthase
Lactosylceramide alpha-2,3-sialyltransferase
Sialyltransferase 9
SIAT9
SIAT9_HUMAN
SIATGM3S
ExpandImages
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Western blot analysis of ST3GAL5 on different lysates with Rabbit anti-ST3GAL5 antibody (HA500547) at 1/2,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: HeLa cell lysate
Lane 3: Jurkat cell lysate
Lane 4: U-87 MG cell lysate
Lane 5: Mouse lung tissue lysate
Lane 6: Mouse skeletal muscle tissue lysate
Lane 7: Rat lung tissue lysate
Lane 8: Rat skeletal muscle tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500547) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"