Actin Rabbit Polyclonal Antibody
Catalog# HA500552
Actin Rabbit Polyclonal Antibody
-
WB
-
Arabidopsis thaliana
-
unconjugated
Overview
Product Name
Actin Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Species Reactivity
Arabidopsis thaliana
Validated Applications
WB
Molecular Weight
Predicted band size: 42 kDa
Positive Control
Oryza sativa lysate, Cotton lysate.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
Target
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Essential component of cell cytoskeleton; plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. This is considered as one of the reproductive actins.
Background References
暂无
Subcellular Location
Cytoplasm, cytoskeleton.
UNIPROT
Synonyms
Actin-11
EC:3.6.4.-
ACT11
T21B14.7
T21B14_108
T23B7.5
At3g12110
Images
-
Western blot analysis of Actin on different lysates with Rabbit anti-Actin antibody (HA500552) at 1/2,000 dilution.
Lane 1: Oryza sativa lysate
Lane 2: Cotton lysate
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: Lane 1: 25 seconds; Lane 2: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500552) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"