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LPHN2 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500555

LPHN2 Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

LPHN2 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human LPHN2 aa 421-470.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 163 kDa

Positive Control

SH-SY5Y cell lysate, HUVEC cell lysate, LNCaP cell lysate.

Conjugation

unconjugated

Reactivity Data

WB
Human
Tested Tested
Mouse
Not Recommended
Rat
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1 % BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

Latrophilin 2 is a protein that in humans is encoded by the ADGRL2 gene. This gene encodes a member of the latrophilin subfamily of G-protein coupled receptors (GPCR). Latrophilins may function in both cell adhesion and signal transduction. In experiments with non-human species, endogenous proteolytic cleavage within a cysteine-rich GPS (G-protein-coupled-receptor proteolysis site) domain resulted in two subunits (a large extracellular N-terminal cell adhesion subunit and a subunit with substantial similarity to the secretin/calcitonin family of GPCRs) being non-covalently bound at the cell membrane. While several transcript variants have been described, the biological validity of only one has been determined.

Background References

1. Tanaka K et al. Latrophilin-2 mediates fluid shear stress mechanotransduction at endothelial junctions. EMBO J. 2024 Aug

2. Kang M et al. Latrophilin-2 Deletion in Cardiomyocyte Disrupts Cell Junction, Leading to D-CMP. Circ Res. 2024 Nov 8

Subcellular Location

Membrane.

UNIPROT

SwissProt: O95490 Human

Synonyms

Adgrl2 antibody

Adhesion G protein-coupled receptor L2 antibody

AGRL2_HUMAN antibody

Calcium-independent alpha-latrotoxin receptor 2 antibody

CIRL-2 antibody

CL2 antibody

KIAA0786 antibody

Latrophilin 1 antibody

Latrophilin 2 antibody

Latrophilin homolog 1 antibody

Expand

Images

  • Western blot analysis of LPHN2 on different lysates with Rabbit anti-LPHN2 antibody (<a href="/products/HA500555" style="font-weight: bold;text-decoration: underline;">HA500555</a>) at 1/2,000 dilution.<br /><br />Lane 1: SH-SY5Y cell lysate<br />Lane 2: HUVEC cell lysate<br />Lane 3: LNCaP cell lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 163 kDa<br />Observed band size: 130 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500555" style="font-weight: bold;text-decoration: underline;">HA500555</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of LPHN2 on different lysates with Rabbit anti-LPHN2 antibody (HA500555) at 1/2,000 dilution.

    Lane 1: SH-SY5Y cell lysate
    Lane 2: HUVEC cell lysate
    Lane 3: LNCaP cell lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 163 kDa
    Observed band size: 130 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500555) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"