TMEM50A Rabbit Polyclonal Antibody
Catalog# HA500556
TMEM50A Rabbit Polyclonal Antibody
-
WB
-
Mouse
-
unconjugated
Overview
Product Name
TMEM50A Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within mouse TMEM50A aa 11-60.
Species Reactivity
Mouse
Validated Applications
WB
Target Molecular Weight
Predicted band size: 17 kDa
Positive Control
Mouse colon tissue lysate, Mouse lung tissue lysate, Mouse kidney tissue lysate, Mouse spleen tissue lysate.
Conjugation
unconjugated
Reactivity Data
| WB | |
|---|---|
| Mouse |
|
| Human |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Transmembrane protein 50A is a protein that in humans is encoded by the TMEM50A gene. This gene is located in the RH gene locus, between the RHD and RHCE genes. The function of its protein product is unknown; however, its sequence has potential transmembrane domains suggesting that it may be an integral membrane protein. Its position between the RH genes suggests that polymorphisms in this gene may be tightly linked to RH haplotypes and may contribute to selective pressure for or against certain RH haplotypes.
Background References
1. Chen H et al. Preliminary study on the function of TMEM50A and its correlation with the RH genes. Transfus Med. 2021 Aug
Subcellular Location
Membrane.
UNIPROT
Synonyms
Small membrane protein 1 antibody
TM50A_HUMAN antibody
Tmem50a antibody
Transmembrane protein 50A antibody
Transmembrane protein 50A
Small membrane protein 1
Tmem50a
Smp1
Images
-
Western blot analysis of TMEM50A on different lysates with Rabbit anti-TMEM50A antibody (HA500556) at 1/1,000 dilution.
Lane 1: Mouse colon tissue lysate
Lane 2: Mouse lung tissue lysate (no heat)
Lane 3: Mouse kidney tissue lysate (no heat)
Lane 4: Mouse spleen tissue lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 30 µg/Lane.
Predicted band size: 17 kDa
Observed band size: 35 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500556) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"