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Repulsive guidance molecule A Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500559

Repulsive guidance molecule A Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Repulsive guidance molecule A Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human RGMA aa 31-440.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 49 kDa

Positive Control

SH-SY5Y cell lysate, HeLa cell lysate.

Conjugation

unconjugated

Reactivity Data

WB
Human
Tested Tested
Mouse
Not Recommended
Rat
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

Repulsive guidance molecule A (RGMa) is a bone morphogenetic protein (BMP) co-receptor of the repulsive guidance molecule family. Together with BMPR1A and BMPR1B, as well as ACVR2A and BMPR2, it binds BMPs thereby activating the intracellular SMAD1/5/8 signalling pathway. In humans this protein is encoded by the RGMA gene. RGMa is a repulsive guidance molecule for retinal axons. Furthermore, neogenin functions as a receptor for RGM. Neogenin overexpression and RGM downexpression in the developing embryonic neural tube induces apoptosis. The apoptotic activity of neogenin in the neural tube is associated with cleavage of its cytoplasmic domain by caspases.

Background References

1. Tang J et al. Repulsive Guidance Molecule-a and Central Nervous System Diseases. Biomed Res Int. 2021 May

2. Isaksen TJ et al. Repulsive Guidance Molecule A Suppresses Adult Neurogenesis. Stem Cell Reports. 2020 Apr

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q96B86 Human

Synonyms

Repulsive guidance molecule A antibody

RGM antibody

RGM domain family member A antibody

RGMA antibody

RGMA_HUMAN antibody

Images

  • Western blot analysis of Repulsive guidance molecule A on different lysates with Rabbit anti-Repulsive guidance molecule A antibody (<a href="/products/HA500559" style="font-weight: bold;text-decoration: underline;">HA500559</a>) at 1/2,000 dilution.<br /><br />Lane 1: SH-SY5Y cell lysate<br />Lane 2: HeLa cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 49 kDa<br />Observed band size: 49 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500559" style="font-weight: bold;text-decoration: underline;">HA500559</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Repulsive guidance molecule A on different lysates with Rabbit anti-Repulsive guidance molecule A antibody (HA500559) at 1/2,000 dilution.

    Lane 1: SH-SY5Y cell lysate
    Lane 2: HeLa cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 49 kDa
    Observed band size: 49 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500559) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"