Skip to content

MDH1B Rabbit Polyclonal Antibody

Usd: 315

Specification

Bulk Order Quote | Customization Request

Catalog# HA500561

MDH1B Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

MDH1B Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within Mouse MDH1B aa 451-500.

Species Reactivity

Mouse

Validated Applications

WB

Molecular Weight

Predicted band size: 56 kDa

Positive Control

Mouse ovary tissue lysate, Mouse testis tissue lysate.

Conjugation

unconjugated

Reactivity Data

WB
Mouse
Tested Tested
Human
Not Recommended
Rat
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:4,000

Target

Function

Predicted to enable L-malate dehydrogenase activity. Predicted to be involved in NADH metabolic process; dicarboxylic acid metabolic process; and tricarboxylic acid cycle.

Background References

暂无

UNIPROT

SwissProt: Q5F204 Mouse

Synonyms

Putative malate dehydrogenase 1B

Mdh1b

Images

  • Western blot analysis of MDH1B on different lysates with Rabbit anti-MDH1B antibody (<a href="/products/HA500561" style="font-weight: bold;text-decoration: underline;">HA500561</a>) at 1/4,000 dilution.<br /><br />Lane 1: Mouse ovary tissue lysate<br />Lane 2: Mouse testis tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 56 kDa<br />Observed band size: 56 kDa<br /><br />Exposure time: 2 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500561" style="font-weight: bold;text-decoration: underline;">HA500561</a>) at 1/4,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MDH1B on different lysates with Rabbit anti-MDH1B antibody (HA500561) at 1/4,000 dilution.

    Lane 1: Mouse ovary tissue lysate
    Lane 2: Mouse testis tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 56 kDa
    Observed band size: 56 kDa

    Exposure time: 2 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500561) at 1/4,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"