DNA helicase HEL308 Rabbit Polyclonal Antibody
Catalog# HA500562
DNA helicase HEL308 Rabbit Polyclonal Antibody
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WB
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Human
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Mouse
-
Rat
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unconjugated
Overview
Product Name
DNA helicase HEL308 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human DNA helicase HEL308 aa 400-450.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 124 kDa
Positive Control
U-2 OS cell lysate, MCF7 cell lysate, HeLa cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate.
Conjugation
unconjugated
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:6,000
Target
Function
Helicase, POLQ-like, also known as Helicase Q (HELQ), HEL308 and Holliday junction migration protein, encoded by the gene HELQ1, is a DNA helicase found in humans, archea and many other organisms. HelQ is a replication-linked repair helicase that preserves DNA integrity through helping in the repair of DNA that has become damaged.
Background References
1. Zhao Y et al. Helicase HELQ: Molecular Characters Fit for DSB Repair Function. Int J Mol Sci. 2024 Aug
2. Zhao Y et al. Human HELQ regulates DNA end resection at DNA double-strand breaks and stalled replication forks. Nucleic Acids Res. 2023 Dec
Subcellular Location
Nucleus, Chromosome.
UNIPROT
Synonyms
HEL308 protein antibody
Helicase POLQ-like antibody
Helicase, POLQ like antibody
HELQ antibody
HELQ_HUMAN antibody
Mus308 like helicase antibody
Mus308-like helicase antibody
POLQ like helicase antibody
POLQ-like helicase antibody
Images
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Western blot analysis of DNA helicase HEL308 on different lysates with Rabbit anti-DNA helicase HEL308 antibody (HA500562) at 1/6,000 dilution.
Lane 1: U-2 OS cell lysate
Lane 2: MCF7 cell lysate
Lane 3: HeLa cell lysate
Lane 4: Mouse testis tissue lysate
Lane 5: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 124 kDa
Observed band size: 124 kDa
Exposure time: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500562) at 1/6,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"