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ELL3 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500564

ELL3 Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

ELL3 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within Mouse ELL3 aa 100-395.

Species Reactivity

Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 45 kDa

Positive Control

Mouse testis tissue lysate, Mouse spleen tissue lysate, Rat testis tissue lysate, Rat spleen tissue lysate.

Conjugation

unconjugated

Reactivity Data

WB
Mouse
Tested Tested
Rat
Tested Tested
Human
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:5,000-1:20,000

Target

Function

Elongation factor that can increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Belongs to the ELL/occludin family. tissue specificity:Testis specific.

Background References

1. Meng S et al. Young LINE-1 transposon 5\' UTRs marked by elongation factor ELL3 function as enhancers to regulate naive pluripotency in embryonic stem cells. Nat Cell Biol. 2023 Sep

2. Kim I et al. Ell3 stimulates 5-FU resistance in a breast cancer cell line. Oncol Lett. 2017 Jun

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q80VR2 Mouse

SwissProt: Q5XFX8 Rat

Synonyms

Elongation Factor RNA Polymerase II-Like 3 antibody

RNA polymerase II elongation factor ELL3 antibody

ELL3

Images

  • Western blot analysis of ELL3 on different lysates with Rabbit anti-ELL3 antibody (<a href="/products/HA500564" style="font-weight: bold;text-decoration: underline;">HA500564</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse testis tissue lysate<br />Lane 2: Mouse spleen tissue lysate<br />Lane 3: Rat testis tissue lysate<br />Lane 4: Rat spleen tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 45 kDa<br />Observed band size: 60 kDa<br /><br />Exposure time: 12 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500564" style="font-weight: bold;text-decoration: underline;">HA500564</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ELL3 on different lysates with Rabbit anti-ELL3 antibody (HA500564) at 1/5,000 dilution.

    Lane 1: Mouse testis tissue lysate
    Lane 2: Mouse spleen tissue lysate
    Lane 3: Rat testis tissue lysate
    Lane 4: Rat spleen tissue lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 45 kDa
    Observed band size: 60 kDa

    Exposure time: 12 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500564) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"