FCN1 / M-Ficolin Rabbit Polyclonal Antibody
Catalog# HA500567
FCN1 / M-Ficolin Rabbit Polyclonal Antibody
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WB
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IF-Cell
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Human
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Mouse
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unconjugated
Overview
Product Name
FCN1 / M-Ficolin Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human FCN1 aa 70-120.
Species Reactivity
Human, Mouse
Validated Applications
WB, IF-Cell
Molecular Weight
Predicted band size: 35 kDa
Positive Control
HeLa cell lysate, NIH/3T3 cell lysate, U-937.
Conjugation
unconjugated
Reactivity Data
| WB | IF-Cell | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
-
1:2,000
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IF-Cell
-
1:50
Target
Function
Extracellular lectin functioning as a pattern-recognition receptor in innate immunity. Binds the sugar moieties of pathogen-associated molecular patterns (PAMPs) displayed on microbes and activates the lectin pathway of the complement system. May also activate monocytes through a G protein-coupled receptor, FFAR2, inducing the secretion of interleukin-8/IL-8. Binds preferentially to 9-O-acetylated 2-6-linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage.
Background References
1. Chen X et al. Identification of FCN1 as a novel macrophage infiltration-associated biomarker for diagnosis of pediatric inflammatory bowel diseases. J Transl Med. 2023 Mar
2. Zhan W et al. Sputum Transcriptomics Reveals FCN1+ Macrophage Activation in Mild Eosinophilic Asthma Compared to Non-Asthmatic Eosinophilic Bronchitis. Allergy Asthma Immunol Res. 2024 Jan
Subcellular Location
Secreted, Cell membrane.
Synonyms
Collagen/fibrinogen domain containing protein 1 antibody
Collagen/fibrinogen domain-containing protein 1 antibody
Fcn1 antibody
FCN1_HUMAN antibody
FCNM antibody
ficolin (collagen/fibrinogen domain containing) 1 antibody
Ficolin 1 antibody
Ficolin A antibody
Ficolin alpha antibody
Ficolin-1 antibody
ExpandImages
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Western blot analysis of FCN1 / M-Ficolin on different lysates with Rabbit anti-FCN1 / M-Ficolin antibody (HA500567) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa cell lysate treated with deglycosylation
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 cell lysate treated with deglycosylation
Lysates/proteins at 20 µg/Lane.
Predicted band size: 35 kDa
Observed band size: 35/70 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500567) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of U-937 cells labeling FCN1 / M-Ficolin with Rabbit anti-FCN1 / M-Ficolin antibody (HA500567) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FCN1 / M-Ficolin antibody (HA500567) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"