PPAR Rabbit Polyclonal Antibody
Catalog# HA500569
PPAR Rabbit Polyclonal Antibody
-
WB
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
PPAR Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human PPARA aa 151-468.
Species Reactivity
Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 52 kDa
Positive Control
Mouse liver tissue lysate, Mouse skeletal muscle tissue lysate, Rat liver tissue lysate, Rat skeletal muscle tissue lysate.
Conjugation
unconjugated
Reactivity Data
| WB | |
|---|---|
| Mouse |
|
| Rat |
|
| Human |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:5,000
Target
Function
In the field of molecular biology, the peroxisome proliferator–activated receptors (PPARs) are a group of nuclear receptor proteins that function as transcription factors regulating the expression of genes. PPARs play essential roles in the regulation of cellular differentiation, development, and metabolism (carbohydrate, lipid, protein), and tumorigenesis of higher organisms. Endogenous ligands for the PPARs include free fatty acids, eicosanoids and Vitamin B3. PPARγ is activated by PGJ2 (a prostaglandin) and certain members of the 5-HETE family of arachidonic acid metabolites including 5-oxo-15(S)-HETE and 5-oxo-ETE. In contrast, PPARα is activated by leukotriene B4. Certain members of the 15-hydroxyeicosatetraenoic acid family of arachidonic acid metabolites, including 15(S)-HETE, 15(R)-HETE, and 15-HpETE activate to varying degrees PPAR alpha, beta/delta, and gamma. In addition, PPARγ was reported to be involved in cancer pathogenesis and growth. PPARγ activation by agonist RS5444 may inhibit anaplastic thyroid cancer growth.
Background References
暂无
Subcellular Location
Nucleus.
UNIPROT
Synonyms
hPPAR antibody
MGC2237 antibody
MGC2452 antibody
NR1C1 antibody
Nuclear receptor subfamily 1 group C member 1 antibody
OTTHUMP00000197740 antibody
OTTHUMP00000197741 antibody
Peroxisome proliferative activated receptor alpha antibody
Peroxisome proliferator activated receptor alpha antibody
Peroxisome proliferator-activated receptor alpha antibody
ExpandImages
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Western blot analysis of PPAR on different lysates with Rabbit anti-PPAR antibody (HA500569) at 1/5,000 dilution.
Lane 1: Mouse liver tissue lysate
Lane 2: Mouse skeletal muscle tissue lysate
Lane 3: Rat liver tissue lysate
Lane 4: Rat skeletal muscle tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 42/52 kDa
Exposure time: 15 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500569) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
-
Design and fabrication of multifunctional cyanidin-3-O-glucoside-loaded nanonutriosomes with enhanced physicochemical stability and hepatic targeting for mitigating alcoholic liver injury
Journal: Chemical Engineering Journal
DOI: 10.1016/j.cej.2026.174791
IF: 13.2
Application: WB
Reactivity: Mouse
Publish date: 2026 Mar