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NDNF Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500571

NDNF Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

NDNF Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within Mouse NDNF aa 20-568.

Species Reactivity

Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 65 kDa

Positive Control

Mouse lung tissue lysate, Mouse brain tissue lysate, Rat lung tissue lysate.

Conjugation

unconjugated

Reactivity Data

WB
Mouse
Tested Tested
Rat
Tested Tested
Human

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:10,000

Target

Function

Secretory protein that plays a role in various cellular processes. Acts as a chemorepellent acting on gonadotropin-releasing hormone (GnRH) expressing neurons regulating their migration to the hypothalamus. Also promotes neuron migration, growth and survival as well as neurite outgrowth and is involved in the development of the olfactory system. May also act through the regulation of growth factors activity and downstream signaling. Also regulates extracellular matrix assembly and cell adhesiveness (By similarity). Promotes endothelial cell survival, vessel formation and plays an important role in the process of revascularization through NOS3-dependent mechanisms.

Background References

1. Cheng W et al. Intrathecal delivery of AAV-NDNF ameliorates disease progression of ALS mice. Mol Ther. 2023 Nov

2. Liebergall SR et al. Ndnf Interneuron Excitability Is Spared in a Mouse Model of Dravet Syndrome. J Neurosci. 2024 Apr

Subcellular Location

Secreted.

UNIPROT

SwissProt: Q8C119 Mouse

Entrez Gene: 312401 Rat

Synonyms

Protein NDNF

Epidermacan

Neuron-derived neurotrophic factor

Ndnf

Images

  • Western blot analysis of NDNF on different lysates with Rabbit anti-NDNF antibody (<a href="/products/HA500571" style="font-weight: bold;text-decoration: underline;">HA500571</a>) at 1/10,000 dilution.<br /><br />Lane 1: Mouse lung tissue lysate<br />Lane 2: Mouse brain tissue lysate<br />Lane 3: Rat lung tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 65 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500571" style="font-weight: bold;text-decoration: underline;">HA500571</a>) at 1/10,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of NDNF on different lysates with Rabbit anti-NDNF antibody (HA500571) at 1/10,000 dilution.

    Lane 1: Mouse lung tissue lysate
    Lane 2: Mouse brain tissue lysate
    Lane 3: Rat lung tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 65 kDa
    Observed band size: 75 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500571) at 1/10,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"