NDNF Rabbit Polyclonal Antibody
Catalog# HA500571
NDNF Rabbit Polyclonal Antibody
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WB
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
NDNF Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Mouse NDNF aa 20-568.
Species Reactivity
Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 65 kDa
Positive Control
Mouse lung tissue lysate, Mouse brain tissue lysate, Rat lung tissue lysate.
Conjugation
unconjugated
Reactivity Data
| WB | |
|---|---|
| Mouse |
|
| Rat |
|
| Human |
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:10,000
Target
Function
Secretory protein that plays a role in various cellular processes. Acts as a chemorepellent acting on gonadotropin-releasing hormone (GnRH) expressing neurons regulating their migration to the hypothalamus. Also promotes neuron migration, growth and survival as well as neurite outgrowth and is involved in the development of the olfactory system. May also act through the regulation of growth factors activity and downstream signaling. Also regulates extracellular matrix assembly and cell adhesiveness (By similarity). Promotes endothelial cell survival, vessel formation and plays an important role in the process of revascularization through NOS3-dependent mechanisms.
Background References
1. Cheng W et al. Intrathecal delivery of AAV-NDNF ameliorates disease progression of ALS mice. Mol Ther. 2023 Nov
2. Liebergall SR et al. Ndnf Interneuron Excitability Is Spared in a Mouse Model of Dravet Syndrome. J Neurosci. 2024 Apr
Subcellular Location
Secreted.
Synonyms
Protein NDNF
Epidermacan
Neuron-derived neurotrophic factor
Ndnf
Images
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Western blot analysis of NDNF on different lysates with Rabbit anti-NDNF antibody (HA500571) at 1/10,000 dilution.
Lane 1: Mouse lung tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat lung tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 75 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500571) at 1/10,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"