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LRG1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500576

LRG1 Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

LRG1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within Mouse LRG1 aa 150-342.

Species Reactivity

Human, Mouse

Validated Applications

WB

Molecular Weight

Predicted band size: 38 kDa

Positive Control

Human plasma tissue lysate, Human serum tissue lysate, Mouse serum tissue lysate, Mouse plasma tissue lysate.

Conjugation

unconjugated

Reactivity Data

WB
Human
Tested Tested
Mouse
Tested Tested
Rat

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:5,000

Target

Function

Leucine-rich alpha-2-glycoprotein 1 is a protein which in humans is encoded by the gene LRG1. The leucine-rich repeat (LRR) family of proteins, including LRG1, have been shown to be involved in protein-protein interaction, signal transduction, and cell adhesion and development. LRG1 is expressed during granulocyte differentiation. LRG1 has been shown to be involved in promoting neovascularization (new blood vessel growth) through causing a switch in transforming growth factor beta (TGFbeta) signaling in endothelial cells. LRG1 binds to the accessory receptor endoglin and promotes signaling via the ALK1-Smad1/5/8 pathway.

Background References

1. Camilli C et al. LRG1: an emerging player in disease pathogenesis. J Biomed Sci. 2022 Jan

2. He S et al. LRG1 is an adipokine that mediates obesity-induced hepatosteatosis and insulin resistance. J Clin Invest. 2021 Dec

Subcellular Location

Secreted.

UNIPROT

SwissProt: P02750 Human

SwissProt: Q91XL1 Mouse

Synonyms

1300008B03Rik antibody

2310031E04Rik antibody

A2GL antibody

A2GL_HUMAN antibody

HMFT1766 antibody

Leucine rich alpha 2 glycoprotein antibody

Leucine-rich alpha-2-glycoprotein 1 antibody

Leucine-rich alpha-2-glycoprotein antibody

Leucine-rich alpha-2-glycoprotein precursor antibody

LRG antibody

Expand

Images

  • Western blot analysis of LRG1 on different lysates with Rabbit anti-LRG1 antibody (<a href="/products/HA500576" style="font-weight: bold;text-decoration: underline;">HA500576</a>) at 1/5,000 dilution.<br /><br />Lane 1: Human plasma tissue lysate<br />Lane 2: Human serum tissue lysate<br />Lane 3: Mouse serum tissue lysate<br />Lane 4: Mouse plasma tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 38 kDa<br />Observed band size: 48 kDa<br /><br />Exposure time: 2 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500576" style="font-weight: bold;text-decoration: underline;">HA500576</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of LRG1 on different lysates with Rabbit anti-LRG1 antibody (HA500576) at 1/5,000 dilution.

    Lane 1: Human plasma tissue lysate
    Lane 2: Human serum tissue lysate
    Lane 3: Mouse serum tissue lysate
    Lane 4: Mouse plasma tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 38 kDa
    Observed band size: 48 kDa

    Exposure time: 2 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500576) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"