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ZCWPW2 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500579

ZCWPW2 Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

ZCWPW2 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within Mouse ZCWPW2 aa 100-331.

Species Reactivity

Mouse

Validated Applications

WB

Molecular Weight

Predicted band size: 38 kDa

Positive Control

Mouse liver tissue lysate, Mouse kidney tissue lysate.

Conjugation

unconjugated

Reactivity Data

WB
Mouse
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:20,000

Target

Function

Histone methylation reader which binds to non-methylated (H3K4me0), monomethylated (H3K4me1), dimethylated (H3K4me2) and trimethylated (H3K4me3) 'Lys-4' on histone H3 (By similarity). The order of binding preference is H3K4me3 > H3K4me2 > H3K4me1 > H3K4me0 (By similarity).

Background References

1. Cavassim MIA et al. PRDM9 losses in vertebrates are coupled to those of paralogs ZCWPW1 and ZCWPW2. Proc Natl Acad Sci U S A. 2022 Mar

UNIPROT

SwissProt: Q08EN7 Mouse

Synonyms

ZCPW2_HUMAN antibody

ZCW2 antibody

ZCWPW2 antibody

Zinc finger CW type with PWWP domain 2 antibody

Zinc finger CW-type PWWP domain protein 2 antibody

Images

  • Western blot analysis of ZCWPW2 on different lysates with Rabbit anti-ZCWPW2 antibody (<a href="/products/HA500579" style="font-weight: bold;text-decoration: underline;">HA500579</a>) at 1/20,000 dilution.<br /><br />Lane 1: Mouse liver tissue lysate<br />Lane 2: Mouse kidney tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 38 kDa<br />Observed band size: 38 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500579" style="font-weight: bold;text-decoration: underline;">HA500579</a>) at 1/20,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ZCWPW2 on different lysates with Rabbit anti-ZCWPW2 antibody (HA500579) at 1/20,000 dilution.

    Lane 1: Mouse liver tissue lysate
    Lane 2: Mouse kidney tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500579) at 1/20,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"