p38 delta / MAPK13 Rabbit Polyclonal Antibody
Catalog# HA500582
p38 delta / MAPK13 Rabbit Polyclonal Antibody
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WB
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Human
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unconjugated
Overview
Product Name
p38 delta / MAPK13 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human p38 delta / MAPK13 aa 251-300.
Product Specificity
This antibody has weak cross-reactivity with p38 gamma/MAPK12.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 42 kDa
Positive Control
A431 cell lysate, Caco-2 cell lysate.
Conjugation
unconjugated
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:5,000
Target
Function
Mitogen-activated protein kinase 13 (MAPK 13), also known as stress-activated protein kinase 4 (SAPK4), is an enzyme that in humans is encoded by the MAPK13 gene. The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is closely related to p38 MAP kinase, both of which can be activated by proinflammatory cytokines and cellular stress. MAP kinase kinases 3, and 6 can phosphorylate and activate this kinase. Transcription factor ATF2, and microtubule dynamics regulator stathmin have been shown to be the substrates of this kinase.
Background References
1. Xing R et al. MAPK13 phosphorylates PHGDH and promotes its degradation via chaperone-mediated autophagy during liver injury. Cell Discov. 2025 Feb
2. Kim J et al. MAPK13 stabilization via m(6)A mRNA modification limits anticancer efficacy of rapamycin. J Biol Chem. 2023 Sep
Subcellular Location
Cytoplasm, cytosol, nucleus.
UNIPROT
Synonyms
Mitogen-activated protein kinase 13
EC:2.7.11.24
MAP kinase 13
MAPK 13
Mitogen-activated protein kinase p38 delta
MAP kinase p38 delta
Stress-activated protein kinase 4
MAPK13
PRKM13
SAPK4
Images
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Western blot analysis of p38 delta / MAPK13 on different lysates with Rabbit anti-p38 delta / MAPK13 antibody (HA500582) at 1/5,000 dilution.
Lane 1: A431 cell lysate
Lane 2: Caco-2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500582) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"