WDR47 Rabbit Polyclonal Antibody
Catalog# HA500584
WDR47 Rabbit Polyclonal Antibody
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WB
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Mouse
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Rat
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unconjugated
Overview
Product Name
WDR47 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human WDR47 aa 601-919.
Species Reactivity
Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 102 kDa
Positive Control
Mouse brain tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | |
|---|---|---|
| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
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1:5,000
Target
Function
WD repeat domain 47 is a protein that in humans is encoded by the WDR47 gene. WDR47 is a prognostic marker in Head and neck squamous cell carcinoma, Kidney renal clear cell carcinoma. Predicted to be located in cytoplasm and microtubule.
Background References
1. Liu H et al. Wdr47, Camsaps, and Katanin cooperate to generate ciliary central microtubules. Nat Commun. 2021 Oct
2. Buijs RR et al. WDR47 protects neuronal microtubule minus ends from katanin-mediated severing. Cell Rep. 2021 Jul
Subcellular Location
Cytoplasm, cytoskeleton.
Synonyms
RGD1309181 antibody
WD repeat containing protein 47 antibody
WD repeat domain 47 antibody
WD repeat-containing protein 47 antibody
Wdr47 antibody
WDR47_HUMAN antibody
Images
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Western blot analysis of WDR47 on different lysates with Rabbit anti-WDR47 antibody (HA500584) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 102 kDa
Observed band size: 110 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500584) at 1/5,000 dilution was used in 5%BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"