Skip to content

WDR47 Rabbit Polyclonal Antibody

Usd: 315

Specification

Bulk Order Quote | Customization Request

Catalog# HA500584

WDR47 Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

WDR47 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human WDR47 aa 601-919.

Species Reactivity

Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 102 kDa

Positive Control

Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Reactivity Data

WB
Mouse
Tested Tested
Rat
Tested Tested
Human
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:5,000

Target

Function

WD repeat domain 47 is a protein that in humans is encoded by the WDR47 gene. WDR47 is a prognostic marker in Head and neck squamous cell carcinoma, Kidney renal clear cell carcinoma. Predicted to be located in cytoplasm and microtubule.

Background References

1. Liu H et al. Wdr47, Camsaps, and Katanin cooperate to generate ciliary central microtubules. Nat Commun. 2021 Oct

2. Buijs RR et al. WDR47 protects neuronal microtubule minus ends from katanin-mediated severing. Cell Rep. 2021 Jul

Subcellular Location

Cytoplasm, cytoskeleton.

UNIPROT

SwissProt: Q8CGF6 Mouse

Entrez Gene: 310785 Rat

Synonyms

RGD1309181 antibody

WD repeat containing protein 47 antibody

WD repeat domain 47 antibody

WD repeat-containing protein 47 antibody

Wdr47 antibody

WDR47_HUMAN antibody

Images

  • Western blot analysis of WDR47 on different lysates with Rabbit anti-WDR47 antibody (<a href="/products/HA500584" style="font-weight: bold;text-decoration: underline;">HA500584</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Rat brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 102 kDa<br />Observed band size: 110 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500584" style="font-weight: bold;text-decoration: underline;">HA500584</a>) at 1/5,000 dilution was used in 5%BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of WDR47 on different lysates with Rabbit anti-WDR47 antibody (HA500584) at 1/5,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Rat brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 102 kDa
    Observed band size: 110 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500584) at 1/5,000 dilution was used in 5%BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"