OTULIN Rabbit Polyclonal Antibody
Catalog# HA500587
OTULIN Rabbit Polyclonal Antibody
-
WB
-
Human
-
Mouse
-
Rat
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Green monkey
-
unconjugated
Overview
Product Name
OTULIN Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human OTULIN aa 1-352.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB
Molecular Weight
Predicted band size: 40 kDa
Positive Control
HeLa cell lysate, MCF7 cell lysate, 786-0 cell lysate, NIH/3T3 cell lysate, COS-1 cell lysate, Mouse brain tissue lysate, Rat testis tissue lysate.
Conjugation
unconjugated
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
| Green Monkey |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:5,000
Target
Function
Deubiquitinase that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response. Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex. Acts as a negative regulator of NF-kappa-B by regulating the activity of the LUBAC complex. OTULIN function is mainly restricted to homeostasis of the LUBAC complex: acts by removing 'Met-1'-linked autoubiquitination of the LUBAC complex, thereby preventing inactivation of the LUBAC complex. Acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis. In myeloid cell, required to prevent unwarranted secretion of cytokines leading to inflammation and autoimmunity by restricting linear polyubiquitin formation. Plays a role in innate immune response by restricting linear polyubiquitin formation on LUBAC complex in response to NOD2 stimulation, probably to limit NOD2-dependent pro-inflammatory signaling.
Background References
1. Verboom L et al. OTULIN in NF-kappaB signaling, cell death, and disease. Trends Immunol. 2021 Jul
2. Dou B et al. OTULIN deficiency: focus on innate immune system impairment. Front Immunol. 2024 May
Subcellular Location
Cytoplasm.
Synonyms
AIPDS antibody
Deubiquitinating enzyme otulin antibody
F105B_HUMAN antibody
Fam105b antibody
Family with sequence similarity 105, member B antibody
FLJ34884 antibody
GUM antibody
OTU deubiquitinase with linear linkage specificity antibody
OTULIN antibody
Protein FAM105B antibody
ExpandImages
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Western blot analysis of OTULIN on different lysates with Rabbit anti-OTULIN antibody (HA500587) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate
Lane 3: 786-0 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: COS-1 cell lysate
Lane 6: Mouse brain tissue lysate
Lane 7: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500587) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"