beta COP Rabbit Polyclonal Antibody
Catalog# HA500588
beta COP Rabbit Polyclonal Antibody
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WB
-
Human
-
Mouse
-
Rat
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unconjugated
Overview
Product Name
beta COP Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human beta COP aa 571-620.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 107 kDa
Positive Control
HeLa cell lysate, HCT 116 cell lysate, 293T cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate.
Conjugation
unconjugated
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:5,000
Target
Function
The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Plays a functional role in facilitating the transport of kappa-type opioid receptor mRNAs into axons and enhances translation of these proteins. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte surface triglyceride lipase (PNPLA2) with the lipid droplet to mediate lipolysis (By similarity).
Background References
1. Kim SS et al. beta-COP Suppresses the Surface Expression of the TREK2. Cells. 2023 May
2. Kim SS et al. beta-COP Regulates TWIK1/TREK1 Heterodimeric Channel-Mediated Passive Conductance in Astrocytes. Cells. 2022 Oct
Subcellular Location
Cytoplasm, Golgi apparatus membrane, Cytoplasmic vesicle, COPI-coated vesicle membrane, Cell membrane, Endoplasmic reticulum-Golgi intermediate compartment.
Synonyms
Beta-coat protein antibody
Beta-COP antibody
betacop antibody
Coatomer beta subunit antibody
Coatomer protein complex subunit beta 1 antibody
Coatomer protein complex subunit beta antibody
Coatomer subunit beta antibody
COPB antibody
COPB_HUMAN antibody
Copb1 antibody
ExpandImages
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Western blot analysis of beta COP on different lysates with Rabbit anti-beta COP antibody (HA500588) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HCT 116 cell lysate
Lane 3: 293T cell lysate
Lane 4: MCF7 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: Mouse liver tissue lysate
Lane 7: Rat liver tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 107 kDa
Observed band size: 100 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500588) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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