PIGR Rabbit Polyclonal Antibody
Catalog# HA500593
PIGR Rabbit Polyclonal Antibody
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WB
-
IHC-P
-
Human
-
unconjugated
Overview
Product Name
PIGR Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human PIGR aa 1-661.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 83 kDa
Positive Control
A431 cell lysate, human colon tissue, human stomach tissue.
Conjugation
unconjugated
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Human |
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|
| Mouse |
|
|
| Rat |
|
|
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:5,000
-
IHC-P
-
1:2,000
Target
Function
Polymeric immunoglobulin receptor (pIgR) is a transmembrane protein that in humans is encoded by the PIGR gene. It is an Fc receptor which facilitates the transcytosis of the soluble polymeric isoforms of immunoglobulin A and immunoglobulin M (pIg) and immune complexes. pIgRs are mainly located on the epithelial lining of mucosal surfaces of the gastrointestinal tract. The composition of the receptor is complex, including 6 immunoglobulin-like domains, a transmembrane region, and an intracellular domain. pIgR expression is under the strong regulation of cytokines, hormones, and pathogenic stimuli.
Background References
1. Chae J et al. Polymeric immunoglobulin receptor (pIgR) in cancer. J Cancer Res Clin Oncol. 2023 Dec
2. Zingoni A et al. PIGR-enriched circulating vesicles contributes to hepatocellular carcinoma aggressiveness. J Hepatol. 2022 Apr
Subcellular Location
Cell membrane, Secreted.
UNIPROT
Synonyms
Hepatocellular carcinoma associated protein TB6 antibody
Hepatocellular carcinoma-associated protein TB6 antibody
MGC125361 antibody
MGC125362 antibody
Phosphatidylinositol glycan, class R antibody
PIGR antibody
PIGR_HUMAN antibody
Poly Ig receptor antibody
Poly-Ig receptor antibody
Polymeric immunoglobulin receptor antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of PIGR on different lysates with Rabbit anti-PIGR antibody (HA500593) at 1/5,000 dilution.
Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (negative) (20 µg/Lane)
Predicted band size: 83 kDa
Observed band size: 83-100 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500593) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-PIGR antibody (HA500593) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500593) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-PIGR antibody (HA500593) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500593) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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