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TMEM184A Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500595

TMEM184A Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

TMEM184A Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human TMEM184A aa 191-240.

Species Reactivity

Human, Mouse

Validated Applications

WB

Molecular Weight

Predicted band size: 46 kDa

Positive Control

HT-29 cell lysate, AGS cell lysate, Mouse kidney tissue lysate.

Conjugation

unconjugated

Reactivity Data

WB
Human
Tested Tested
Mouse
Tested Tested
Rat
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:20,000

Target

Function

Acts as a heparin receptor in vascular cells (By similarity). May be involved in vesicle transport in exocrine cells and Sertoli cells (By similarity). Predicted to enable heparin binding activity. Predicted to act upstream of or within germ-line sex determination; regulation of protein localization; and regulation of secretion. Predicted to be located in cytoplasmic vesicle; perinuclear region of cytoplasm; and plasma membrane. Predicted to be active in early endosome membrane. Predicted to be integral component of membrane.

Background References

1. Altenburg LM et al. Transmembrane Protein-184A Interacts with Syndecan-4 and Rab GTPases and Is Required to Maintain VE-Cadherin Levels. Cells. 2025 Jun

2. Field CJ et al. Involvement of transmembrane protein 184a during angiogenesis in zebrafish embryos. Front Physiol. 2022 Sep

Subcellular Location

Cell membrane, Cytoplasm, perinuclear region, Cytoplasmic vesicle membrane, Early endosome membrane, Endosome, Cytoplasmic vesicle, secretory vesicle membrane.

UNIPROT

SwissProt: Q6ZMB5 Human

SwissProt: Q3UFJ6 Mouse

Synonyms

Transmembrane protein 184A

TMEM184A

Images

  • Western blot analysis of TMEM184A on different lysates with Rabbit anti-TMEM184A antibody (<a href="/products/HA500595" style="font-weight: bold;text-decoration: underline;">HA500595</a>) at 1/20,000 dilution.<br /><br />Lane 1: HT-29 cell lysate<br />Lane 2: AGS cell lysate<br />Lane 3: Mouse kidney tissue lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 46 kDa<br />Observed band size: 46 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500595" style="font-weight: bold;text-decoration: underline;">HA500595</a>) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TMEM184A on different lysates with Rabbit anti-TMEM184A antibody (HA500595) at 1/20,000 dilution.

    Lane 1: HT-29 cell lysate
    Lane 2: AGS cell lysate
    Lane 3: Mouse kidney tissue lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 46 kDa
    Observed band size: 46 kDa

    Exposure time: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500595) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"