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MICA Mouse Monoclonal Antibody [A4C5]

Usd: 350

Specification

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Catalog# HA600002

MICA Mouse Monoclonal Antibody [A4C5]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

MICA Mouse Monoclonal Antibody [A4C5]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human MICA aa 200-300.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 43 kDa

Positive Control

MCF-7 cell lysate, Hela cell lysate.

Conjugation

unconjugated

Clone Number

A4C5

RRID

AB_3071620

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

Target

Function

MHC class I polypeptide–related sequence A (MICA) is a highly polymorphic cell surface glycoprotein encoded by the MICA gene located within MHC locus. MICA is related to MHC class I and it has similar domain structure, however, it is not associated with β2-microglobulin nor binds peptides as conventional MHC class I molecules do. MICA rather functions as a stress-induced ligand (as a danger signal) for integral membrane protein receptor NKG2D ("natural-killer group 2, member D"). MICA is broadly recognized by NK cells, γδ T cells, and CD8+ αβ T cells which carry NKG2D receptor on their cell surface and which are activated via this interaction.

Background References

1. Groh V. et. al. Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells. Science 279:1737-1740(1998).

Subcellular Location

Cell membrane, Cytoplasm, Membrane.

UNIPROT

SwissProt: Q29983 Human

Synonyms

HLA class I antigen antibody

FLJ36918 antibody

FLJ60820 antibody

MGC111087 antibody

MGC21250 antibody

MHC class I chain related gene A protein antibody

MHC class I chain related protein A antibody

MHC class I chain related protein A HLA B HLA C antibody

MHC class I polypeptide related sequence A antibody

MHC class I polypeptide-related sequence A antibody

Expand

Images

  • Western blot analysis of MICA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/HA600002" style="font-weight: bold;text-decoration: underline;">HA600002</a>, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: MCF-7 cell lysate<br />Lane 2: Hela cell lysate

    Western blot analysis of MICA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600002, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: MCF-7 cell lysate
    Lane 2: Hela cell lysate

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"