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Trop2 Recombinant Antibody [A5C5] - Human IgG1 (Chimeric)

Usd: 350

Specification

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Catalog# HA600011

Trop2 Recombinant Antibody [A5C5] - Human IgG1 (Chimeric)

Application

  • ELISA

  • FC(Intra)

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Trop2 Recombinant Antibody [A5C5] - Human IgG1 (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Recombinant protein within Human TROP2 aa27-274 (Extracellular) in HEK293 cell.

Product Specificity

This Human Recombinant monoclonal chimeric antibody has been engineered from a Mouse parent antibody. By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other Mouse-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.

Species Reactivity

Human

Validated Applications

ELISA, FC(Intra)

Molecular Weight

35.7 kDa

Positive Control

Recombinant TROP2 protein, MDA-MB-468 cell.

Conjugation

unconjugated

Clone Number

A5C5

RRID

AB_3071629

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein G affinity purified.

Application Dilution

  • ELISA

  • 1:5,000-1:20,000

  • FC(Intra)

  • 1:100-1:200

Target

Function

Tumor-associated calcium signal transducer 2, also known as Trop-2 and as epithelial glycoprotein-1 antigen (EGP-1), is a protein that in humans is encoded by the TACSTD2 gene. This intronless gene encodes a carcinoma-associated antigen defined by the monoclonal antibody GA733. This antigen is a member of a family including at least two type I membrane proteins. It transduces an intracellular calcium signal and acts as a cell surface receptor. Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness. This antigen is the target of sacituzumab govitecan, an antibody-drug conjugate. TROP-2 is related to epithelial cell adhesion molecule (EpCAM), also called TROP-1, gp40, and KSA. Trop-1 and Trop-2 are homologous to serum IGF-II-binding proteins and appear as signal transducers. Thus, they likely represent novel cell-surface receptors and may play a role in regulating the growth of carcinoma cells. This human monoclonal chimeric antibody has been engineered from the mouse monoclonal parent antibody. By necessity, some mouse sequence is retained as part of the variable domain. When multiplexing with other mouse-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

Background References

1. Anthony Simms et al. TROP-2 expression in papillary thyroid carcinoma: Potential Diagnostic Utility. Diagn Cytopathol. 2016 Jan;44(1):26-31.

Subcellular Location

Membrane.

UNIPROT

SwissProt: P09758 Human

Synonyms

Cell surface glycoprotein Trop 2 antibody

Cell surface glycoprotein Trop-2 antibody

Cell surface glycoprotein Trop2 antibody

Epithelial glycoprotein 1 antibody

GA733 1 antibody

GA7331 antibody

M1S 1 antibody

M1S1 antibody

Membrane component chromosome 1 surface marker 1 antibody

Pancreatic carcinoma marker protein GA733 1 antibody

Expand

Images

  • The binding activity of <a href="/products/HA600011" style="font-weight: bold;text-decoration: underline;">HA600011</a> with recombinant TROP2 protein.<br />Immobilized recombinant TROP2 protein at 1 ug/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (<a href="/products/HA600011" style="font-weight: bold;text-decoration: underline;">HA600011</a>) for 1 hour at 25℃. Donkey anti human IgG-AP at 1/1000 dilution was used as the secondary antibody. The EC50 of <a href="/products/HA600011" style="font-weight: bold;text-decoration: underline;">HA600011</a> is 6.958 ng/ml.

    The binding activity of HA600011 with recombinant TROP2 protein.
    Immobilized recombinant TROP2 protein at 1 ug/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600011) for 1 hour at 25℃. Donkey anti human IgG-AP at 1/1000 dilution was used as the secondary antibody. The EC50 of HA600011 is 6.958 ng/ml.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed MDA-MB-468 (Human breast adenocarcinoma cell line) cells labeling TROP2 with <a href="/products/HA600011" style="font-weight: bold;text-decoration: underline;">HA600011</a> at 1/200 dilution (red) compared with Purified human IgG, Isotype Control (blue). Goat anti human IgG-FITC at 1/1000 dilution was used as the secondary antibody.

    Flow cytometric analysis of 4% paraformaldehyde-fixed MDA-MB-468 (Human breast adenocarcinoma cell line) cells labeling TROP2 with HA600011 at 1/200 dilution (red) compared with Purified human IgG, Isotype Control (blue). Goat anti human IgG-FITC at 1/1000 dilution was used as the secondary antibody.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation