Trop2 Recombinant Antibody [A5C6] - Human IgG1 (Chimeric)
Catalog# HA600012
Trop2 Recombinant Antibody [A5C6] - Human IgG1 (Chimeric)
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ELISA
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FC(Intra)
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Human
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unconjugated
Overview
Product Name
Trop2 Recombinant Antibody [A5C6] - Human IgG1 (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Recombinant protein within Human TROP2 aa27-274 (Extracellular) in HEK293 cell.
Product Specificity
This Human Recombinant monoclonal chimeric antibody has been engineered from a Mouse parent antibody. By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other Mouse-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.
Species Reactivity
Human
Validated Applications
ELISA, FC(Intra)
Molecular Weight
35.7 kDa
Positive Control
Recombinant TROP2 protein, MDA-MB-468 cell.
Conjugation
unconjugated
Clone Number
A5C6
RRID
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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ELISA
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1:5,000-1:20,000
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FC(Intra)
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1:100-1:200
Target
Function
Tumor-associated calcium signal transducer 2, also known as Trop-2 and as epithelial glycoprotein-1 antigen (EGP-1), is a protein that in humans is encoded by the TACSTD2 gene. This intronless gene encodes a carcinoma-associated antigen defined by the monoclonal antibody GA733. This antigen is a member of a family including at least two type I membrane proteins. It transduces an intracellular calcium signal and acts as a cell surface receptor. Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness. This antigen is the target of sacituzumab govitecan, an antibody-drug conjugate. TROP-2 is related to epithelial cell adhesion molecule (EpCAM), also called TROP-1, gp40, and KSA. Trop-1 and Trop-2 are homologous to serum IGF-II-binding proteins and appear as signal transducers. Thus, they likely represent novel cell-surface receptors and may play a role in regulating the growth of carcinoma cells. This human monoclonal chimeric antibody has been engineered from the mouse monoclonal parent antibody. By necessity, some mouse sequence is retained as part of the variable domain. When multiplexing with other mouse-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
Background References
1. Anthony Simms et al. TROP-2 expression in papillary thyroid carcinoma: Potential Diagnostic Utility. Diagn Cytopathol. 2016 Jan;44(1):26-31.
Subcellular Location
Membrane.
UNIPROT
Synonyms
Cell surface glycoprotein Trop 2 antibody
Cell surface glycoprotein Trop-2 antibody
Cell surface glycoprotein Trop2 antibody
Epithelial glycoprotein 1 antibody
GA733 1 antibody
GA7331 antibody
M1S 1 antibody
M1S1 antibody
Membrane component chromosome 1 surface marker 1 antibody
Pancreatic carcinoma marker protein GA733 1 antibody
ExpandImages
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The binding activity of HA600012 with Recombinant TROP2 protein.
Immobilized Recombinant TROP2 protein at 1 ug/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600012) for 1 hour at 25℃. Donkey anti human IgG-AP at 1/1000 dilution was used as the secondary antibody. The EC50 of HA600012 is 5.574 ng/ml. -
Flow cytometric analysis of 4% paraformaldehyde-fixed MDA-MB-468 (Human breast adenocarcinoma cell line) cells labeling TROP2 with HA600012 at 1/200 dilution (red) compared with Purified human IgG, Isotype Control (black). Goat anti human IgG-FITC at 1/1000 dilution was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"