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IREB2 Mouse Monoclonal Antibody [9D1]

Usd: 350

Specification

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Catalog# HA600015

IREB2 Mouse Monoclonal Antibody [9D1]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

IREB2 Mouse Monoclonal Antibody [9D1]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human IREB2 aa 1-963.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 105 kDa

Positive Control

Jurkat cell lysate, A549 cell lysate, Mouse liver tissue lysate, Mouse kidney tissue lysate, Rat liver tissue lysate, human placenta tissue.

Conjugation

unconjugated

Clone Number

9D1

RRID

AB_3071633

Product Features

Form

Liquid

Concentration

2 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:100-1:500

Target

Function

RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.

Background References

1. Samaniego F. et. al. Molecular characterization of a second iron-responsive element binding protein, iron regulatory protein 2. Structure, function, and post-translational regulation. J. Biol. Chem. 269:30904-30910(1994).

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P48200 Human

SwissProt: Q811J3 Mouse

SwissProt: Q62751 Rat

Synonyms

ACO3 antibody

FLJ23381 antibody

IRE BP2 antibody

IRE-BP 2 antibody

IREB 2 antibody

IREB2 antibody

IREB2_HUMAN antibody

Iron regulatory protein 2 antibody

iron responsive element binding protein 2 antibody

Iron-responsive element-binding protein 2 antibody

Expand

Images

  • Western blot analysis of IREB2 on different lysates with Mouse anti-IREB2 antibody (<a href="/products/HA600015" style="font-weight: bold;text-decoration: underline;">HA600015</a>) at 1/2,000 dilution.<br /><br />Lane 1: Jurkat cell lysate (20 µg/Lane)<br />Lane 2: A549 cell lysate (20 µg/Lane)<br />Lane 3: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 4: Mouse kidney tissue lysate (40 µg/Lane)<br />Lane 5: Rat liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 105 kDa<br />Observed band size: 105 kDa<br /><br />Exposure time: 11 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA600015" style="font-weight: bold;text-decoration: underline;">HA600015</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP, AlpSdAbs&reg; VHH(HRP) (<a href="/products/001-100-005" style="font-weight: bold;text-decoration: underline;">001-100-005</a>) at 1/5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of IREB2 on different lysates with Mouse anti-IREB2 antibody (HA600015) at 1/2,000 dilution.

    Lane 1: Jurkat cell lysate (20 µg/Lane)
    Lane 2: A549 cell lysate (20 µg/Lane)
    Lane 3: Mouse liver tissue lysate (40 µg/Lane)
    Lane 4: Mouse kidney tissue lysate (40 µg/Lane)
    Lane 5: Rat liver tissue lysate (40 µg/Lane)

    Predicted band size: 105 kDa
    Observed band size: 105 kDa

    Exposure time: 11 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600015) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP, AlpSdAbs® VHH(HRP) (001-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-IREB2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA600015" style="font-weight: bold;text-decoration: underline;">HA600015</a>, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-IREB2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600015, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Citation

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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