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NSUN4 Mouse Monoclonal Antibody [4G4]

Usd: 350

Specification

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Catalog# HA600023

NSUN4 Mouse Monoclonal Antibody [4G4]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

NSUN4 Mouse Monoclonal Antibody [4G4]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human NSUN4 aa 1-384.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 43 kDa

Positive Control

MCF7 cell lysate, HEK-293 cell lysate, K-562 cell lysate, HepG2 cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, mouse testis tissue, human prostate tissue.

Conjugation

unconjugated

Clone Number

4G4

RRID

AB_3071640

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:100-1:500

Target

Function

Involved in mitochondrial ribosome assembly. 5-methylcytosine rRNA methyltransferase that probably is involved in mitochondrial ribosome small subunit (SSU) maturation by methylation of mitochondrial 12S rRNA; the function is independent of MTERFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU). Targeted to LSU by MTERFD2/MTERF4 and probably is involved in a final step in ribosome biogenesis to ensure that SSU and LSU are assembled. In vitro can methylate 16S rRNA of the LSU; the methylation is enhanced by MTERFD/MTERF4.

Background References

1. Camara Y. et. al. MTERF4 regulates translation by targeting the methyltransferase NSUN4 to the mammalian mitochondrial ribosome. Cell Metab. 13:527-539(2011).

Subcellular Location

Mitochondrion.

UNIPROT

SwissProt: Q96CB9 Human

SwissProt: Q9CZ57 Mouse

Synonyms

5 methylcytosine rRNA methyltransferase NSUN4 antibody

EC=2.1.1.- antibody

NOL1/NOP2/Sun domain family member 4 antibody

NOL1/NOP2/Sun domain family, member 4 antibody

NOP2/Sun domain family, member 4 antibody

Nsun4 antibody

NSUN4_HUMAN antibody

Putative methyltransferase NSUN4 antibody

RP4-603I14.2 antibody

Shtap antibody

Expand

Images

  • Western blot analysis of NSUN4 on different lysates with Mouse anti-NSUN4 antibody (<a href="/products/HA600023" style="font-weight: bold;text-decoration: underline;">HA600023</a>) at 1/500 dilution.<br /><br />Lane 1: MCF7 cell lysate<br />Lane 2: HEK-293 cell lysate<br />Lane 3: K-562 cell lysate<br />Lane 4: HepG2 cell lysate<br />Lane 5: HeLa cell lysate<br />Lane 6: SH-SY5Y cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 43 kDa<br />Observed band size: 43 kDa<br /><br />Exposure time: 1 minute 2 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA600023" style="font-weight: bold;text-decoration: underline;">HA600023</a>) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of NSUN4 on different lysates with Mouse anti-NSUN4 antibody (HA600023) at 1/500 dilution.

    Lane 1: MCF7 cell lysate
    Lane 2: HEK-293 cell lysate
    Lane 3: K-562 cell lysate
    Lane 4: HepG2 cell lysate
    Lane 5: HeLa cell lysate
    Lane 6: SH-SY5Y cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

    Exposure time: 1 minute 2 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600023) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-NSUN4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA600023" style="font-weight: bold;text-decoration: underline;">HA600023</a>, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-NSUN4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600023, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-NSUN4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA600023" style="font-weight: bold;text-decoration: underline;">HA600023</a>, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-NSUN4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600023, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"