Angiotensin II Type 1 Receptor Mouse Monoclonal Antibody [11B3]
Catalog# HA600029
Angiotensin II Type 1 Receptor Mouse Monoclonal Antibody [11B3]
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WB
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IF-Cell
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Angiotensin II Type 1 Receptor Mouse Monoclonal Antibody [11B3]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within human Angiotensin II Type 1 Receptor aa 300-350.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, FC
Target Molecular Weight
Predicted band size: 41 kDa
Positive Control
PC-12 cell lysate, A549 cell lysate, HepG2 cell lysate, HeLa cell lysate, PC-12, 293T, A549.
Conjugation
unconjugated
Clone Number
11B3
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:100
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FC
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1:50-1:100
Target
Function
The AT1 receptor mediates the major cardiovascular effects of angiotensin II. Effects include vasoconstriction, aldosterone synthesis and secretion, increased vasopressin secretion, cardiac hypertrophy, augmentation of peripheral noradrenergic activity, vascular smooth muscle cells proliferation, decreased renal blood flow, renal renin inhibition, renal tubular sodium reuptake, modulation of central sympathetic nervous system activity, cardiac contractility, central osmocontrol and extracellular matrix formation.
Background References
1. Jia W. et. al. A strategy for precise and large scale identification of core fucosylated glycoproteins. Mol. Cell. Proteomics 8:913-923(2009).
Subcellular Location
Cell membrane, Membrane.
Synonyms
AG2S antibody
Agtr 1 antibody
Agtr1 antibody
AGTR1_HUMAN antibody
Agtr1a antibody
AGTR1B antibody
Ang II antibody
Angiotensin II receptor type 1 antibody
Angiotensin II type-1 receptor antibody
Angiotensin receptor 1 antibody
ExpandImages
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Western blot analysis of Angiotensin II Type 1 Receptor on different lysates with Mouse anti-Angiotensin II Type 1 Receptor antibody (HA600029) at 1/500 dilution.
Lane 1: PC-12 cell lysate
Lane 2: A549 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: HeLa cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 41 kDa
Observed band size: 49 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600029) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/200,000 dilution was used for 1 hour at room temperature. -
ICC staining of Angiotensin II Type 1 Receptor in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA600029, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of Angiotensin II Type 1 Receptor in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA600029, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Flow cytometric analysis of Angiotensin II Type 1 Receptor was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA600029, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"