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Angiotensin II Type 1 Receptor Mouse Monoclonal Antibody [11B3]

Usd: 350

Specification

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Catalog# HA600029

Angiotensin II Type 1 Receptor Mouse Monoclonal Antibody [11B3]

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Angiotensin II Type 1 Receptor Mouse Monoclonal Antibody [11B3]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within human Angiotensin II Type 1 Receptor aa 300-350.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, FC

Molecular Weight

Predicted band size: 41 kDa

Positive Control

PC-12 cell lysate, A549 cell lysate, HepG2 cell lysate, HeLa cell lysate, PC-12, 293T, A549.

Conjugation

unconjugated

Clone Number

11B3

RRID

AB_3071646

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:50-1:100

  • FC

  • 1:50-1:100

Target

Function

The AT1 receptor mediates the major cardiovascular effects of angiotensin II. Effects include vasoconstriction, aldosterone synthesis and secretion, increased vasopressin secretion, cardiac hypertrophy, augmentation of peripheral noradrenergic activity, vascular smooth muscle cells proliferation, decreased renal blood flow, renal renin inhibition, renal tubular sodium reuptake, modulation of central sympathetic nervous system activity, cardiac contractility, central osmocontrol and extracellular matrix formation.

Background References

1. Jia W. et. al. A strategy for precise and large scale identification of core fucosylated glycoproteins. Mol. Cell. Proteomics 8:913-923(2009).

Subcellular Location

Cell membrane, Membrane.

UNIPROT

SwissProt: P30556 Human

SwissProt: P25095 Rat

SwissProt: P29089 Rat

Synonyms

AG2S antibody

Agtr 1 antibody

Agtr1 antibody

AGTR1_HUMAN antibody

Agtr1a antibody

AGTR1B antibody

Ang II antibody

Angiotensin II receptor type 1 antibody

Angiotensin II type-1 receptor antibody

Angiotensin receptor 1 antibody

Expand

Images

  • Western blot analysis of Angiotensin II Type 1 Receptor on different lysates with Mouse anti-Angiotensin II Type 1 Receptor antibody (<a href="/products/HA600029" style="font-weight: bold;text-decoration: underline;">HA600029</a>) at 1/500 dilution.<br /><br />Lane 1: PC-12 cell lysate<br />Lane 2: A549 cell lysate<br />Lane 3: HepG2 cell lysate<br />Lane 4: HeLa cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 41 kDa<br />Observed band size: 49 kDa<br /><br />Exposure time: 2 minutes;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA600029" style="font-weight: bold;text-decoration: underline;">HA600029</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Angiotensin II Type 1 Receptor on different lysates with Mouse anti-Angiotensin II Type 1 Receptor antibody (HA600029) at 1/500 dilution.

    Lane 1: PC-12 cell lysate
    Lane 2: A549 cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: HeLa cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 41 kDa
    Observed band size: 49 kDa

    Exposure time: 2 minutes;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600029) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/200,000 dilution was used for 1 hour at room temperature.

  • ICC staining of Angiotensin II Type 1 Receptor in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/HA600029" style="font-weight: bold;text-decoration: underline;">HA600029</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Angiotensin II Type 1 Receptor in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA600029, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • ICC staining of Angiotensin II Type 1 Receptor in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/HA600029" style="font-weight: bold;text-decoration: underline;">HA600029</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Angiotensin II Type 1 Receptor in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA600029, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • Flow cytometric analysis of Angiotensin II Type 1 Receptor was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/HA600029" style="font-weight: bold;text-decoration: underline;">HA600029</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Angiotensin II Type 1 Receptor was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA600029, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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