MMAF Mouse Monoclonal Antibody [A5G11]
Catalog# HA600035
MMAF Mouse Monoclonal Antibody [A5G11]
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ELISA
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Species independent
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unconjugated
Overview
Product Name
MMAF Mouse Monoclonal Antibody [A5G11]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
MMAF coupled with OVA.
Species Reactivity
Species independent
Validated Applications
ELISA
Conjugation
unconjugated
Clone Number
A5G11
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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ELISA
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1:5,000-1:20,000
Target
Function
Monomethyl auristatin F (MMAF) is a synthetic antineoplastic agent. It is part of the approved drug belantamab mafodotin in multiple myeloma and some experimental anti-cancer antibody-drug conjugates such as vorsetuzumab mafodotin and SGN-CD19A. In International Nonproprietary Names for MMAF-antibody-conjugates, the name mafodotin refers to MMAF plus its attachment structure to the antibody. Monomethyl auristatin F is an antimitotic agent which inhibits cell division by blocking the polymerisation of tubulin. It is linked to an antibody with high affinity to structures on cancer cells, causing MMAF to accumulate in such cells. MMAF is actually desmethyl-auristatin F; that is, the N-terminal amino group has only one methyl substituent instead of two as in auristatin F itself.
Background References
1. Yu-Tzu Tai. et. al. Novel anti–B-cell maturation antigen antibody-drug conjugate (GSK2857916) selectively induces killing of multiple myeloma.Blood. 2014 May 15; 123(20): 3128–3138. doi: 10.1182/blood-2013-10-535088. PMID: 24569262.
Synonyms
MMAF antibody
Monomethyl auristatin F antibdy
Images
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The binding activity of HA600035 with MMAF-ADC, MMAF-BSA, MMAE-BSA, DM1-ADC and BSA protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600035) for 1 hour at 25℃.
Mouse anti-MMAF Ab (HA600035) can bind to MMAF-ADC or MMAF-BSA but not BSA or MMAE-BSA. -
Down ELISA Binding Assay of anti-MMAF Antibody A5G11(HA600035) to MMAF-ADC and MMAF-BSA. The mouse mAb works fine with ELISA assay for measuring MMAF derivative ADC.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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