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MMAF Mouse Monoclonal Antibody [A5G11]

Usd: 185

Specification

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Catalog# HA600035

MMAF Mouse Monoclonal Antibody [A5G11]

Application

  • ELISA

Reactivity

  • Species independent

Conjugation

  • unconjugated

Overview

Product Name

MMAF Mouse Monoclonal Antibody [A5G11]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

MMAF coupled with OVA.

Species Reactivity

Species independent

Validated Applications

ELISA

Conjugation

unconjugated

Clone Number

A5G11

RRID

AB_3071652

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4)

Isotype

IgG1

Purification Method

Protein G affinity purified.

Application Dilution

  • ELISA

  • 1:5,000-1:20,000

Target

Function

Monomethyl auristatin F (MMAF) is a synthetic antineoplastic agent. It is part of the approved drug belantamab mafodotin in multiple myeloma and some experimental anti-cancer antibody-drug conjugates such as vorsetuzumab mafodotin and SGN-CD19A. In International Nonproprietary Names for MMAF-antibody-conjugates, the name mafodotin refers to MMAF plus its attachment structure to the antibody. Monomethyl auristatin F is an antimitotic agent which inhibits cell division by blocking the polymerisation of tubulin. It is linked to an antibody with high affinity to structures on cancer cells, causing MMAF to accumulate in such cells. MMAF is actually desmethyl-auristatin F; that is, the N-terminal amino group has only one methyl substituent instead of two as in auristatin F itself.

Background References

1. Yu-Tzu Tai. et. al. Novel anti–B-cell maturation antigen antibody-drug conjugate (GSK2857916) selectively induces killing of multiple myeloma.Blood. 2014 May 15; 123(20): 3128–3138. doi: 10.1182/blood-2013-10-535088. PMID: 24569262.

Synonyms

MMAF antibody

Monomethyl auristatin F antibdy

Images

  • The binding activity of <a href="/products/HA600035" style="font-weight: bold;text-decoration: underline;">HA600035</a> with MMAF-ADC, MMAF-BSA, MMAE-BSA, DM1-ADC and BSA protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (<a href="/products/HA600035" style="font-weight: bold;text-decoration: underline;">HA600035</a>) for 1 hour at 25℃.<br /><br />Mouse anti-MMAF Ab (<a href="/products/HA600035" style="font-weight: bold;text-decoration: underline;">HA600035</a>) can bind to MMAF-ADC or MMAF-BSA but not BSA or MMAE-BSA.

    The binding activity of HA600035 with MMAF-ADC, MMAF-BSA, MMAE-BSA, DM1-ADC and BSA protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600035) for 1 hour at 25℃.

    Mouse anti-MMAF Ab (HA600035) can bind to MMAF-ADC or MMAF-BSA but not BSA or MMAE-BSA.

  • Down ELISA Binding Assay of anti-MMAF Antibody A5G11(<a href="/products/HA600035" style="font-weight: bold;text-decoration: underline;">HA600035</a>) to MMAF-ADC and MMAF-BSA. The mouse mAb works fine with ELISA assay for measuring MMAF derivative ADC.

    Down ELISA Binding Assay of anti-MMAF Antibody A5G11(HA600035) to MMAF-ADC and MMAF-BSA. The mouse mAb works fine with ELISA assay for measuring MMAF derivative ADC.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"