Strep-Tag II Mouse Monoclonal Antibody [A5D10]
Catalog# HA600037
Strep-Tag II Mouse Monoclonal Antibody [A5D10]
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WB
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Species independent
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unconjugated
Overview
Product Name
Strep-Tag II Mouse Monoclonal Antibody [A5D10]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide corresponding to Strep-tag II.
Species Reactivity
Species independent
Validated Applications
WB
Conjugation
unconjugated
Clone Number
A5D10
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
Target
Function
The Strep-tag® system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag II is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin®, a specifically engineered streptavidin, and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins.
Background References
1. Arne Skerra. et. al. The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins. Nat Protoc. 2007;2(6):1528-35.
Synonyms
anti-Strep-Tag II antibody
Strep-Tag II antibody
Strep-Tag II antibody antibody
Strep-Tag IIantiboy antibody
Images
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Western blot analysis of Strep-Tag II on different lysates with Mouse anti-Strep-Tag II antibody (HA600037) at 1/1,000 dilution.
Lane 1: 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate
Lane 2: 293T transfected with Strep-Tag II-tagged ACAT2 (N-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600037) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"