PSMA Mouse Monoclonal Antibody [A5F12]
Catalog# HA600046
PSMA Mouse Monoclonal Antibody [A5F12]
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
PSMA Mouse Monoclonal Antibody [A5F12]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human PSMA aa 30-750/750.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 84 kDa
Positive Control
LNCaP cell lysate, 22RV1 cell lysate, human prostate tissue, human prostate carcinoma tissue.
Conjugation
unconjugated
Clone Number
A5F12
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:200
Target
Function
This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms.
Background References
1. Maurer T. et. al. Current use of PSMA-PET in prostate cancer management. Nat Rev Urol. 2016 Apr
2. Emmett L. et. al. Lutetium (177) PSMA radionuclide therapy for men with prostate cancer: a review of the current literature and discussion of practical aspects of therapy. J Med Radiat Sci. 2017 Mar
Subcellular Location
Cell membrane, Cytoplasm.
UNIPROT
Synonyms
Cell growth inhibiting protein 27 antibody
Cell growth-inhibiting gene 27 protein antibody
FGCP antibody
Folate hydrolase (prostate-specific membrane antigen) 1 antibody
Folate hydrolase 1 antibody
Folate hydrolase antibody
Folate hydrolase prostate specific membrane antigen 1 antibody
FOLH 1 antibody
FOLH antibody
Folh1 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of PSMA on different lysates with Mouse anti-PSMA antibody (HA600046) at 1/1,000 dilution.
Lane 1: LNCaP cell lysate
Lane 2: 22RV1 cell lysate
Lane 3: PC-3M cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 84 kDa
Observed band size: 100/200 kDa
Exposure time: 15 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600046) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-PSMA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600046, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-PSMA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600046, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"