RUNX3 Mouse Monoclonal Antibody [A7B7]
Catalog# HA600058
RUNX3 Mouse Monoclonal Antibody [A7B7]
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WB
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IHC-P
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Human
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Rat
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unconjugated
Overview
Product Name
RUNX3 Mouse Monoclonal Antibody [A7B7]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human RUNX3 aa 151-350/415.
Species Reactivity
Human, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 44 kDa
Positive Control
SW620 cell lysate, Ramos cell lysate, Raji cell lysates, rat large intestine tissue, human tonsils tissue, human colon tissue.
Conjugation
unconjugated
Clone Number
A7B7
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IHC-P
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1:200-1:600
Target
Function
Runt-related transcription factor 3 is a protein that in humans is encoded by the RUNX3 gene. This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-YGYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene. In melanocytic cells RUNX3 gene expression may be regulated by MITF. Most of this literature used RUNX3 promoter methylation status in various cancers as a proxy for its expression. However, quite many genes are known to be methylated in tumor cell genomes, and the majority of these genes are not expressed in the normal tissue of origin of these cancers.
Background References
1. Date Y. et. al. Oncogenic RUNX3: A Link between p53 Deficiency and MYC Dysregulation. Mol Cells. 2020 Feb
2. Kumar A. et. al. UnPAKing RUNX3 functions-Both sides of the coin. Small GTPases. 2019 Jul
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
Acute myeloid leukemia 2 protein antibody
Acute myeloid leukemia gene 2 antibody
AML 2 antibody
AML2 antibody
CBF alpha 3 antibody
CBF-alpha-3 antibody
CBFA 3 antibody
CBFA3 antibody
Core binding factor alpha 3 subunit antibody
core binding factor antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of RUNX3 on different lysates with Mouse anti-RUNX3 antibody (HA600058) at 1/5,000 dilution.
Lane 1: SW620 cell lysate
Lane 2: MCF7 cell lysate (negative)
Lane 3: Ramos cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 44/46 kDa
Exposure time: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600058) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of RUNX3 on Raji cell lysates with Mouse anti-RUNX3 antibody (HA600058) at 1/2,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600058) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Mouse anti-RUNX3 antibody (HA600058) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600058) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsils tissue with Mouse anti-RUNX3 antibody (HA600058) at 1/600 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600058) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-RUNX3 antibody (HA600058) at 1/600 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600058) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"