ACVR2B Mouse Monoclonal Antibody [A5H7]
Catalog# HA600067
ACVR2B Mouse Monoclonal Antibody [A5H7]
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WB
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Human
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Mouse
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Rat
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Zebrafish
Predicted species support after-sales service
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unconjugated
Overview
Product Name
ACVR2B Mouse Monoclonal Antibody [A5H7]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within human ACVR2B aa 331-380 / 512.
Species Reactivity
Human (Predicted: Mouse, Rat, Zebrafish)
Predicted species support after-sales service
Validated Applications
WB
Molecular Weight
58 kDa
Positive Control
Hela cell lysates.
Conjugation
unconjugated
Clone Number
A5H7
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2a
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:500-1:2,000
Target
Function
Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptors are considered to be constitutively active kinases. This gene encodes activin A type IIB receptor, which displays a 3- to 4-fold higher affinity for the ligand than activin A type II receptor.
Background References
1. Huot JR. et. al. ACVR2B antagonism as a countermeasure to multi-organ perturbations in metastatic colorectal cancer cachexia. J Cachexia Sarcopenia Muscle. 2020 Dec
2. Szabó Z. et. al. Systemic blockade of ACVR2B ligands attenuates muscle wasting in ischemic heart failure without compromising cardiac function. FASEB J. 2020 Aug
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
Activin A receptor type IIB antibody
Activin receptor type 2B antibody
Activin receptor type IIB antibody
Activin receptor type-2B antibody
ActR IIB antibody
ACTR-IIB antibody
ActRIIB antibody
ACVR 2B antibody
ACVR2B antibody
AVR2B_HUMAN antibody
ExpandImages
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Western blot analysis of ACVR2B on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600067, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
Predicted band size: 58 kDa
Observed band size: 50 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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