CX3CR1 Mouse Monoclonal Antibody [A5C7]
Catalog# HA600074
CX3CR1 Mouse Monoclonal Antibody [A5C7]
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IF-Cell
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Human
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unconjugated
Overview
Product Name
CX3CR1 Mouse Monoclonal Antibody [A5C7]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within human CX3CR1 aa 1-50 / 355.
Species Reactivity
Human
Validated Applications
IF-Cell
Molecular Weight
40 kDa
Positive Control
MCF-7.
Conjugation
unconjugated
Clone Number
A5C7
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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IF-Cell
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1:50-1:200
Target
Function
Fractalkine is a transmembrane protein and chemokine involved in the adhesion and migration of leukocytes. The protein encoded by this gene is a receptor for fractalkine. The encoded protein also is a coreceptor for HIV-1, and some variations in this gene lead to increased susceptibility to HIV-1 infection and rapid progression Receptor for the C-X3-C chemokine fractalkine (CX3CL1) present on many early leukocyte cells; CX3CR1-CX3CL1 signaling exerts distinct functions in different tissue compartments, such as immune response, inflammation, cell adhesion and chemotaxis. CX3CR1-CX3CL1 signaling mediates cell migratory functions. Responsible for the recruitment of natural killer (NK) cells to inflamed tissues. Acts as a regulator of inflammation process leading to atherogenesis by mediating macrophage and monocyte recruitment to inflamed atherosclerotic plaques, promoting cell survival. Involved in airway inflammation by promoting interleukin 2-producing T helper (Th2) cell survival in inflamed lung. Involved in the migration of circulating monocytes to non-inflamed tissues, where they differentiate into macrophages and dendritic cells. Acts as a negative regulator of angiogenesis, probably by promoting macrophage chemotaxis. Plays a key role in brain microglia by regulating inflammatory response in the central nervous system (CNS) and regulating synapse maturation. Required to restrain the microglial inflammatory response in the CNS and the resulting parenchymal damage in response to pathological stimuli. Involved in brain development by participating to synaptic pruning, a natural process during which brain microglia eliminates extra synapses during postnatal development. Synaptic pruning by microglia is required to promote the maturation of circuit connectivity during brain development.AIDS. Four transcript variants encoding two different isoforms have been found for this gene.
Background References
1. Pawelec P. et. al. The Impact of the CX3CL1/CX3CR1 Axis in Neurological Disorders. Cells. 2020 Oct
2. Gyoneva S. et. al. Cx3cr1-deficient microglia exhibit a premature aging transcriptome. Life Sci Alliance. 2019 Dec
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
Beta chemokine receptor-like 1 antibody
C X3 C CKR 1 antibody
C-X3-C CKR-1 antibody
CCRL1 antibody
Chemokine C X3 C motif receptor 1 antibody
CMK BRL 1 antibody
CMK-BRL-1 antibody
CMK-BRL1 antibody
CMKBLR1 antibody
CMKDR1 antibody
ExpandImages
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ICC staining of CX3CR1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA600074, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"