Mouse anti-Human IgG Mouse Monoclonal Antibody [A5D12]
Catalog# HA600076
Mouse anti-Human IgG Mouse Monoclonal Antibody [A5D12]
-
WB
-
ELISA
-
Human
-
unconjugated
Overview
Product Name
Mouse anti-Human IgG Mouse Monoclonal Antibody [A5D12]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Full length native protein corresponding to Human IgG.
Species Reactivity
Human
Validated Applications
WB, ELISA
Positive Control
Human IgG protein.
Conjugation
unconjugated
Clone Number
A5D12
RRID
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein G affinity purified.
Application Dilution
-
WB
-
1:500-1:2,000
-
ELISA
-
1:1,000-1:20,000
Target
Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.
Background References
暂无
Subcellular Location
Cell Membrane and Secreted.
UNIPROT
Synonyms
FLJ39988 antibody
FLJ40036 antibody
FLJ40253 antibody
FLJ40587 antibody
FLJ40789 antibody
FLJ40834 antibody
G1m marker antibody
G2m marker antibody
G3m marker antibody
G4m marker antibody
ExpandImages
-
Western blot analysis of Mouse anti-Human IgG on different lysates with Mouse anti-Mouse anti-Human IgG antibody (HA600076) at 1/1,000 dilution.
Lane 1: Recombinant human IgG protein
Lane 2: Recombinant human IgM protein
Lysates/proteins at 50 ng/Lane.
Observed band size: 50 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600076) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immobilized Human IgG protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600076) for 1 hour at 25℃.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"