AMPK alpha 2 Mouse Monoclonal Antibody [A6A10]

Western blot analysis of AMPK alpha 2 on HeLa and Jurkat cell lysates with Mouse anti-AMPK alpha 2 antibody (HA600078) at 1:1,000 dilution.


Lysates/proteins at 10 µg/Lane.
Exposure time: 60 seconds;


Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA600078, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat Anti-Mouse IgG-HRP(HA1006), 1/100,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 62.3 kDa
Observed band size: 62 kDa

Western blot analysis of AMPK alpha 2 on NIH/3T3 cell lysates with Mouse anti-AMPK alpha 2 antibody (HA600078) at 1:2,000 dilution.


Lysates/proteins at 10 µg/Lane.
Exposure time: 20 seconds;


Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA600078, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat Anti-Mouse IgG-HRP(HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 62.3 kDa
Observed band size: 62 kDa

Western blot analysis of AMPK alpha 2 on PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600078, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.

ICC staining of AMPK alpha 2 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA600078, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AMPK alpha 2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA600078, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AMPK alpha 2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA600078, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-AMPK alpha 2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA600078, 1/500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry analysis of PC-12 cells labeling AMPK alpha 2 with Mouse anti-AMPK alpha 2 antibody (HA600078) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-AMPK alpha 2 antibody (HA600078) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.