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AMPK alpha 2 Mouse Monoclonal Antibody [A6A12]

Usd: 350

Specification

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Catalog# HA600080

AMPK alpha 2 Mouse Monoclonal Antibody [A6A12]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

AMPK alpha 2 Mouse Monoclonal Antibody [A6A12]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human AMPK alpha 2 aa 231-430.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 62 kDa

Positive Control

HeLa cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Rat liver tissue lysate.

Conjugation

unconjugated

Clone Number

A6A12

RRID

AB_3071696

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Background References

1. Zhang Q. et. al. miR-4999-5p Predicts Colorectal Cancer Survival Outcome and Reprograms Glucose Metabolism by Targeting PRKAA2. Onco Targets Ther. 2020 Feb

2. Wu J. et. al. MicroRNA-27a Suppresses Detrusor Fibrosis in Streptozotocin-Induced Diabetic Rats by Targeting PRKAA2 Through the TGF-beta1/Smad3 Signaling Pathway. Cell Physiol Biochem. 2018

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: P54646 Human

SwissProt: Q8BRK8 Mouse

SwissProt: Q09137 Rat

Synonyms

5'-AMP-activated protein kinase catalytic subunit alpha-2 antibody

AAPK2_HUMAN antibody

ACACA kinase antibody

Acetyl CoA carboxylase kinase antibody

Acetyl-CoA carboxylase kinase antibody

AMPK alpha 2 chain antibody

AMPK subunit alpha-2 antibody

AMPK2 antibody

AMPKa2 antibody

AMPKalpha2 antibody

Expand

Images

  • Western blot analysis of AMPK alpha 2 on different lysates with Mouse anti-AMPK alpha 2 antibody (<a href="/products/HA600080" style="font-weight: bold;text-decoration: underline;">HA600080</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: HEK-293 cell lysate (20 µg/Lane)<br />Lane 3: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 4: C6 cell lysate (20 µg/Lane)<br />Lane 5: Rat liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 62 kDa<br />Observed band size: 62 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA600080" style="font-weight: bold;text-decoration: underline;">HA600080</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of AMPK alpha 2 on different lysates with Mouse anti-AMPK alpha 2 antibody (HA600080) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: HEK-293 cell lysate (20 µg/Lane)
    Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 4: C6 cell lysate (20 µg/Lane)
    Lane 5: Rat liver tissue lysate (40 µg/Lane)

    Predicted band size: 62 kDa
    Observed band size: 62 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600080) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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