Prolactin Receptor / PRL-R Mouse Monoclonal Antibody [A6E4]
Catalog# HA600099
Prolactin Receptor / PRL-R Mouse Monoclonal Antibody [A6E4]
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WB
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IF-Cell
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
Prolactin Receptor / PRL-R Mouse Monoclonal Antibody [A6E4]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human Prolactin Receptor aa 150-350 / 622.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P, FC
Molecular Weight
Predicted band size: 70 kDa
Positive Control
293T cell lysate, Hela cell lysate, MCF-7, human kidney tissue, human pancreas tissue, HeLa.
Conjugation
unconjugated
Clone Number
A6E4
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500
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IF-Cell
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1:100
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IHC-P
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1:200
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FC
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1:1,000
Target
Function
The prolactin receptor (PRLR) is a type I cytokine receptor encoded in humans by the PRLR gene on chromosome 5p13-14. It is the receptor for prolactin (PRL). The PRLR can also bind to and be activated by growth hormone (GH) and human placental lactogen (hPL). The PRLR is expressed in the mammary glands, pituitary gland, and other tissues. It plays an important role in lobuloalveolar development of the mammary glands during pregnancy and in lactation. The PRLR is a class 1 cytokine receptor that uses messenger pathways to control cell proliferation, migration, intracellular ion concentration and inhibit programmed cell death (apoptosis). Expression of the PRLR protein is found within cells of the mammary glands[10] in accordance with its role in lactation, but also is the subject of attention for its diverse and emerging roles by its expression in adipose tissue, pancreatic islet cell proliferation, and immune responses. The PRLR has been found to be essential for lobuloalveolar maturation of the mammary glands during pregnancy, as evidenced by the fact that PRLR knockout mice show severely impaired development of lobuloalveolar structures. Disruption of PRLR signaling pathways have been linked to tumorigenesis and breast cancer development.
Background References
1. Shemanko CS. Prolactin receptor in breast cancer: marker for metastatic risk. J Mol Endocrinol. 2016 Nov
2. Abramicheva PA. et. al. Prolactin Receptor Isoforms as the Basis of Tissue-Specific Action of Prolactin in the Norm and Pathology. Biochemistry (Mosc). 2019 Apr
Subcellular Location
Membrane, Secreted.
UNIPROT
Synonyms
AI987712 antibody
CLONE SPM213 antibody
CPRLP antibody
Delta 4-delta 7/11 truncated prolactin receptor antibody
Delta 4-SF1b truncated prolactin receptor antibody
HPRL antibody
hPRL receptor antibody
hPRLrI antibody
Lactogen receptor antibody
MFAB antibody
ExpandImages
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Western blot analysis of Prolactin Receptor / PRL-R on different lysates with Mouse anti-Prolactin Receptor / PRL-R antibody (HA600099) at 1/500 dilution.
Lane 1: 293T cell lysate (10 µg/Lane)
Lane 2: Hela cell lysate (10 µg/Lane)
Predicted band size: 70 kDa
Observed band size: 100 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600099) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of MCF-7 cells labeling Prolactin Receptor / PRL-R with Mouse anti-Prolactin Receptor / PRL-R antibody (HA600099) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Prolactin Receptor / PRL-R antibody (HA600099) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Prolactin Receptor / PRL-R antibody (HA600099) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600099) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-Prolactin Receptor / PRL-R antibody (HA600099) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600099) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of HeLa cells labeling Prolactin Receptor / PRL-R.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA600099, 1/1,000) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"