iFluor™ 647 Conjugated pan Cytokeratin Recombinant Mouse Monoclonal Antibody [PD00-15]
Catalog# HA600105F
iFluor™ 647 Conjugated pan Cytokeratin Recombinant Mouse Monoclonal Antibody [PD00-15]
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IF-Tissue
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FC
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Human
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iFluor™ 647
Overview
Product Name
iFluor™ 647 Conjugated pan Cytokeratin Recombinant Mouse Monoclonal Antibody [PD00-15]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Synthetic peptide.
Species Reactivity
Human
Validated Applications
IF-Tissue, FC
Positive Control
Human breast tissue, MCF-7.
Conjugation
iFluor™ 647
Clone Number
PD00-15
RRID
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| FC | IF-Cell | IF-Tissue | |
|---|---|---|---|
| human |
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|
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Tissue
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1:200
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FC
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1:500-1:1,000
Target
Function
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. For example, cytokeratins 10 and 13 are expressed highly in a subset of squamous cell carcinomas while cytokeratin 18 is expressed in a majority of adenocarcinomas and basal cell carcinomas. Since each epithelium contains at least one acidic and one basic keratin, this antibody is used to observe the distribution of keratin-containing cells in normal epithelia and to identify neoplasms derived from such epithelium. Recognizes human cytokeratins 4, 5, 6, 8, 10, 13, and 18.
Background References
1. Vranes V et al. Analysis of Spatial Distribution and Prognostic Value of Different Pan Cytokeratin Immunostaining Intensities in Breast Tumor Tissue Sections. Int J Mol Sci. 2020 Jun
2. Sidey K et al. Identifying squamous cell carcinoma and cytokeratin-derived amyloid with pan-cytokeratin AE1/AE3 during Mohs micrographic surgery. JAAD Case Rep. 2020 Jun
Subcellular Location
Cytoplasm.
Synonyms
pan ck antibody
pan-ck antibody
panck antibody
pan Keratin antibody
Images
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Immunofluorescence analysis of paraffin-embedded human breast tissue labeling pan Cytokeratin (HA600105F).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody pan Cytokeratin (HA600105F, iFluor™ 647) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. -
Flow cytometric analysis of MCF-7 cells labeling pan Cytokeratin.
Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with pan Cytokeratin (HA600105F, iFluor™ 647, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"