APC Conjugated Human CD8 alpha Recombinant Mouse Monoclonal Antibody [PSH04-94]
Catalog# HA600124F
APC Conjugated Human CD8 alpha Recombinant Mouse Monoclonal Antibody [PSH04-94]
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FC
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Human
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APC
Overview
Product Name
APC Conjugated Human CD8 alpha Recombinant Mouse Monoclonal Antibody [PSH04-94]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human CD8a aa 22-182.
Species Reactivity
Human
Validated Applications
FC
Molecular Weight
Predicted band size: 26 kDa
Positive Control
Human peripheral blood lymphocytes.
Conjugation
APC
Clone Number
PSH04-94
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at 2℃ to 8℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
Supplied in phosphate-buffered solution, pH 7.2, containing 0.2% ProClean 950 and BSA.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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FC
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5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Target
Function
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen-presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain isoforms. Two alternative transcripts encoding distinct isoforms, one membrane associated and one secreted, have been identified.
Background References
1. Zheng Z et al. CD8A as a Prognostic and Immunotherapy Predictive Biomarker Can Be Evaluated by MRI Radiomics Features in Bladder Cancer. Cancers (Basel). 2022 Oct
2. Tanemoto S et al. Single-cell transcriptomics of human gut T cells identifies cytotoxic CD4(+)CD8A(+) T cells related to mouse CD4 cytotoxic T cells. Front Immunol. 2022 Oct
Subcellular Location
Cell membrane; Secreted.
UNIPROT
Synonyms
alpha polypeptide (p32) antibody
CD_antigen=CD8a antibody
CD8 antibody
CD8 antigen alpha polypeptide antibody
CD8 antigen alpha polypeptide (p32) antibody
CD8 antigen, alpha polypeptide (p32) antibody
CD8a antibody
CD8A antigen antibody
CD8A molecule antibody
CD8A_HUMAN antibody
ExpandImages
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Flow cytometric analysis of human peripheral blood lymphocytes labeling Human CD8 alpha.
Cells were washed twice with cold PBS and resuspend. Then incubated for 1 hour at +4℃ with Human CD8 alpha (HA600124F, red, 2μg/mL). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
APC-Cy7 Conjugated Human CD8 alpha Recombinant Mouse Monoclonal Antibody [PSH04-94]
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Human CD8 alpha Recombinant Mouse Monoclonal Antibody [PSH04-94]
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Reactivity: Human
Conjugate: unconjugated
