iFluor™ 488 Conjugated pan Cytokeratin Recombinant Mouse Monoclonal Antibody [PDH09-10]
Catalog# HA600135F
iFluor™ 488 Conjugated pan Cytokeratin Recombinant Mouse Monoclonal Antibody [PDH09-10]
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IF-Cell
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IF-Tissue
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Human
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iFluor™ 488
Overview
Product Name
iFluor™ 488 Conjugated pan Cytokeratin Recombinant Mouse Monoclonal Antibody [PDH09-10]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Full length native protein corresponding to Human pan Cytokeratin.
Species Reactivity
Human
Validated Applications
IF-Cell, IF-Tissue
Positive Control
A431, human small intestine tissue.
Conjugation
iFluor™ 488
Clone Number
PDH09-10
Reactivity Data
| IF-Cell | IF-Tissue | |
|---|---|---|
| Human |
|
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Cell
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1:100
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IF-Tissue
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1:200
Target
Function
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. For example, cytokeratins 10 and 13 are expressed highly in a subset of squamous cell carcinomas while cytokeratin 18 is expressed in a majority of adenocarcinomas and basal cell carcinomas. Since each epithelium contains at least one acidic and one basic keratin, this antibody is used to observe the distribution of keratin-containing cells in normal epithelia and to identify neoplasms derived from such epithelium. Recognizes humancytokeratins 10, 14, 15, 16 and 19, and cytokeratins 1, 2, 3, 4, 5, 6, 7 and 8.
Background References
暂无
Subcellular Location
Cytoplasmic
Synonyms
pan Cytokeratin antibody
pan-Cytokeratin antibody
pan ck antibody
pan-ck antibody
panck antibody
pan Keratin antibody
Images
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Immunocytochemistry analysis of A431 cells labeling pan Cytokeratin with Mouse anti-pan Cytokeratin antibody (HA600135F) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunofluorescence analysis of paraffin-embedded human small intestine tissue labeling pan Cytokeratin with Mouse anti-pan Cytokeratin antibody (HA600135F) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA600135F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"