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SIN1 Mouse Monoclonal Antibody [A6G10]

Usd: 350

Specification

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Catalog# HA601008

SIN1 Mouse Monoclonal Antibody [A6G10]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

SIN1 Mouse Monoclonal Antibody [A6G10]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human SIN1 aa 350-522.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 59 kDa

Positive Control

HeLa cell lysate, A549 cell lysate, MCF7 cell lysate, U-87 MG cell lysate.

Conjugation

unconjugated

Clone Number

A6G10

RRID

AB_3071732

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex.

Background References

1. Huang Y. et. al. Sin1 promotes proliferation and invasion of prostate cancer cells by modulating mTORC2-AKT and AR signaling cascades. Life Sci. 2020 May

2. Xu H. et. al. Nitidine Chloride Inhibits SIN1 Expression in Osteosarcoma Cells. Mol Ther Oncolytics. 2019 Feb

Subcellular Location

Nucleus, Cell membrane, Cytoplasmic vesicle.

UNIPROT

SwissProt: Q9BPZ7 Human

Synonyms

MAPKAP 1 antibody

MAPKAP1 antibody

MEKK2 interacting protein 1 antibody

MGC2745 antibody

MIP 1 antibody

MIP1 antibody

Mitogen activated protein kinase associated protein 1 antibody

Mitogen-activated protein kinase 2-associated protein 1 antibody

mSIN1 antibody

OTTHUMP00000064207 antibody

Expand

Images

  • Western blot analysis of SIN1 on different lysates with Mouse anti-SIN1 antibody (<a href="/products/HA601008" style="font-weight: bold;text-decoration: underline;">HA601008</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: A549 cell lysate<br />Lane 3: MCF7 cell lysate<br />Lane 4: U-87 MG cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 59 kDa<br />Observed band size: 65/75 kDa<br /><br />Exposure time: 24 seconds; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601008" style="font-weight: bold;text-decoration: underline;">HA601008</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SIN1 on different lysates with Mouse anti-SIN1 antibody (HA601008) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: A549 cell lysate
    Lane 3: MCF7 cell lysate
    Lane 4: U-87 MG cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 59 kDa
    Observed band size: 65/75 kDa

    Exposure time: 24 seconds; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601008) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation