VGLUT1 Mouse Monoclonal Antibody [A7A3]
Catalog# HA601014
VGLUT1 Mouse Monoclonal Antibody [A7A3]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
VGLUT1 Mouse Monoclonal Antibody [A7A3]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within Human VGluT1 aa 501-550 / 560.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 62 kDa
Positive Control
Mouse brain tissue lysate, Rat brain tissue lysate, Human brain tissue lysate.
Conjugation
unconjugated
Clone Number
A7A3
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG3
Purification Method
Protein G affinity purified.
Application Dilution
-
WB
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1:500-1:2,000
Target
Function
Vesicular glutamate transporter 1 (VGLUT1) is a protein that in humans is encoded by the SLC17A7 gene. The protein encoded by this gene is a vesicle-bound, sodium-dependent phosphate transporter that is specifically expressed in the neuron-rich regions of the brain. It is preferentially associated with the membranes of synaptic vesicles and functions in glutamate transport. The protein shares 82% identity with the differentiation-associated Na-dependent inorganic phosphate cotransporter and they appear to form a distinct class within the Na+/Pi cotransporter family.
Background References
1. Zha X. et. al. VMHvl-Projecting Vglut1+ Neurons in the Posterior Amygdala Gate Territorial Aggression. Cell Rep. 2020 Apr
2. Du X. et. al. Research progress on the role of type I vesicular glutamate transporter (VGLUT1) in nervous system diseases. Cell Biosci. 2020 Mar
Subcellular Location
Synaptic vesicle membrane, Membrane, Synaptosome.
Synonyms
BNPI antibody
Brain specific Na (+) dependent inorganic phosphate cotransporter antibody
Brain specific Na dependent inorganic phosphate cotransporter antibody
Brain-specific Na(+)-dependent inorganic phosphate cotransporter antibody
Slc17a7 antibody
Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antibody
Solute carrier family 17 member 7 antibody
Vesicular glutamate transporter 1 antibody
VGLU1_HUMAN antibody
VGLUT 1 antibody
ExpandImages
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Western blot analysis of VGLUT1 on different lysates with Mouse anti-VGLUT1 antibody (HA601014) at 1/1,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lane 3: Human brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 62 kDa
Observed band size: 52 kDa
Exposure time: 1 minute 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601014) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"