Topoisomerase II alpha Mouse Monoclonal Antibody [A3F12] (HA601028)
Catalog# HA601028
Mouse Monoclonal to Topoisomerase II alpha
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WB
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IHC-P
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Human
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Mouse
Lane 1: A549 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: NIH/3T3 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 174 kDa
Observed band size: 174 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601028) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
Topoisomerase II alpha Mouse Monoclonal Antibody [A3F12] (HA601028)
Immunogen
Synthetic peptide within human topoisomerase ii alpha aa1480-1531.
Host
Mouse
Positive Control
A549 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, human breast carcinoma tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A3F12
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IHC-P
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein G affinity purified.
Molecular Weight
174/178/182 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
Topoisomerase II alpha
SYNONYMS
alpha isozyme antibody;ATP hydrolyzing DNA topoisomerase II alfa antibody;DNA gyrase antibody;DNA topoisomerase (ATP hydrolyzing) antibody;DNA topoisomerase 2 alpha antibody;DNA topoisomerase 2-alpha antibody;DNA topoisomerase II 170 kD antibody;DNA topoisomerase II alpha isozyme antibody;DNA topoisomerase II antibody;DNA Topoisomerase2 antibody;TOP 2A antibody;TOP2 antibody;TOP2A antibody;TOP2A_HUMAN antibody;Topoisomerase DNA II alpha 170kDa antibody;TP2A antibody
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
DNA topoisomerase IIα is a human enzyme encoded by the TOP2A gene. Topoisomerase IIα relives topological DNA stress during transcription, condenses chromosomes, and separates chromatids. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several chemotherapy agents and a variety of mutations in this gene have been associated with the development of drug resistance.[citation needed] Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. TOP2A has been shown to interact with SMURF2, HDAC1, CDC5L, Small ubiquitin-related modifier 1 and P53.
CITATIONS
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Quantitative acetylome analysis reveals involvement of glucosyltransferase acetylation in Streptococcus mutans biofilm formation
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WB
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Citation
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