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MUM1 Mouse Monoclonal Antibody [A7D1]

Usd: 350

Specification

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Catalog# HA601030

MUM1 Mouse Monoclonal Antibody [A7D1]

Application

  • IHC-P

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

MUM1 Mouse Monoclonal Antibody [A7D1]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within human MUM1 aa 402-451/451.

Species Reactivity

Human

Validated Applications

IHC-P, WB

Molecular Weight

Predicted band size: 52 kDa

Positive Control

Ramos cell lysate, human colon tissue, human lymph nodes tissue.

Conjugation

unconjugated

Clone Number

A7D1

RRID

AB_3071754

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein G affinity purified.

Application Dilution

  • IHC-P

  • 1:1,500-1:2,000

  • WB

  • 1:2,000

Target

Function

MUM1 belongs to the IRF gene family containing at least 10 widely expressed genes with similar DNA binding motif all involved in regulation of cell growth, transformation and induction of apoptosis as well as development of T-cell immune response. MUM1 plays an important role in the regulation of gene expression in response to interferon and other cytokines. MUM1 is a gene primarily identified in Multiple Myeloma cell line where it was localized in region of chromosomal translocations t(6;14)(p25;q32) placing this gene in proximity of IgH enhancer locus. Expression is also important for the differentiation of monocytes along macrophage and dendritic cell pathways. The expression of MUM1 protein appears at the later stages of B-cell differentiation after the expression of CD10 and Bcl-6. B-lymphocytes in light zone of germinal centers (late stage of germinal center differentiation) and post-germinal lymphocytes are generally positive. The expression of MUM1 and Bcl-6 in normal germinal center B cells appears to be mutually exclusive. MUM1 is constantly found at all stages of differentiation of plasma cells. Nuclear expression is present also in a subpopulation of activated T- lymphocytes. MUM1 protein is furthermore expressed in normal and neoplastic melanocytes but not found in other cell types. Some oncogenic viruses (HTLV-I and EBV), activate the NF-?B pathway and consequently elevate MUM1/IRF4 expression.

Background References

1. Alvisi G. et. al. IRF4 instructs effector Treg differentiation and immune suppression in human cancer. J Clin Invest. 2020 Jun

2. Al Mamun A. et. al. Microglial IRF5-IRF4 regulatory axis regulates neuroinflammation after cerebral ischemia and impacts stroke outcomes. Proc Natl Acad Sci U S A. 2020 Jan

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q15306 Human

Synonyms

Interferon regulatory factor 4 antibody

IRF 4 antibody

IRF-4 antibody

Irf4 antibody

IRF4_HUMAN antibody

LSIRF antibody

Lymphocyte specific interferon regulatory factor antibody

Lymphocyte specific IRF antibody

Lymphocyte-specific interferon regulatory factor antibody

Multiple myeloma oncogene 1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of MUM1 on different lysates with Mouse anti-MUM1 antibody (<a href="/products/HA601030" style="font-weight: bold;text-decoration: underline;">HA601030</a>) at 1/2,000 dilution.<br /><br />Lane 1: Ramos cell lysate<br />Lane 2: Jurkat cell lysate (negative)<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 52 kDa<br />Observed band size: 52 kDa<br /><br />Exposure time: 1 minute 22 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601030" style="font-weight: bold;text-decoration: underline;">HA601030</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of MUM1 on different lysates with Mouse anti-MUM1 antibody (HA601030) at 1/2,000 dilution.

    Lane 1: Ramos cell lysate
    Lane 2: Jurkat cell lysate (negative)

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa

    Exposure time: 1 minute 22 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601030) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-MUM1 antibody (<a href="/products/HA601030" style="font-weight: bold;text-decoration: underline;">HA601030</a>) at 1/1,500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601030" style="font-weight: bold;text-decoration: underline;">HA601030</a>) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-MUM1 antibody (HA601030) at 1/1,500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601030) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Mouse anti-MUM1 antibody (<a href="/products/HA601030" style="font-weight: bold;text-decoration: underline;">HA601030</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601030" style="font-weight: bold;text-decoration: underline;">HA601030</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Mouse anti-MUM1 antibody (HA601030) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601030) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"