MUM1 Mouse Monoclonal Antibody [A7C12]
Catalog# HA601031
MUM1 Mouse Monoclonal Antibody [A7C12]
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IHC-P
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Human
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unconjugated
Overview
Product Name
MUM1 Mouse Monoclonal Antibody [A7C12]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within human MUM1 aa 402-451/451.
Species Reactivity
Human
Validated Applications
IHC-P
Molecular Weight
Predicted band size: 52 kDa
Positive Control
Human colon tissue, human lymph nodes tissue.
Conjugation
unconjugated
Clone Number
A7C12
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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IHC-P
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1:2,000
Target
Function
MUM1 belongs to the IRF gene family containing at least 10 widely expressed genes with similar DNA binding motif all involved in regulation of cell growth, transformation and induction of apoptosis as well as development of T-cell immune response. MUM1 plays an important role in the regulation of gene expression in response to interferon and other cytokines. MUM1 is a gene primarily identified in Multiple Myeloma cell line where it was localized in region of chromosomal translocations t(6;14)(p25;q32) placing this gene in proximity of IgH enhancer locus. Expression is also important for the differentiation of monocytes along macrophage and dendritic cell pathways. The expression of MUM1 protein appears at the later stages of B-cell differentiation after the expression of CD10 and Bcl-6. B-lymphocytes in light zone of germinal centers (late stage of germinal center differentiation) and post-germinal lymphocytes are generally positive. The expression of MUM1 and Bcl-6 in normal germinal center B cells appears to be mutually exclusive. MUM1 is constantly found at all stages of differentiation of plasma cells. Nuclear expression is present also in a subpopulation of activated T- lymphocytes. MUM1 protein is furthermore expressed in normal and neoplastic melanocytes but not found in other cell types. Some oncogenic viruses (HTLV-I and EBV), activate the NF-?B pathway and consequently elevate MUM1/IRF4 expression.
Background References
1. Alvisi G. et. al. IRF4 instructs effector Treg differentiation and immune suppression in human cancer. J Clin Invest. 2020 Jun
2. Al Mamun A. et. al. Microglial IRF5-IRF4 regulatory axis regulates neuroinflammation after cerebral ischemia and impacts stroke outcomes. Proc Natl Acad Sci U S A. 2020 Jan
Subcellular Location
Nucleus.
UNIPROT
Synonyms
Interferon regulatory factor 4 antibody
IRF 4 antibody
IRF-4 antibody
Irf4 antibody
IRF4_HUMAN antibody
LSIRF antibody
Lymphocyte specific interferon regulatory factor antibody
Lymphocyte specific IRF antibody
Lymphocyte-specific interferon regulatory factor antibody
Multiple myeloma oncogene 1 antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-MUM1 antibody (HA601031) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601031) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Mouse anti-MUM1 antibody (HA601031) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601031) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"