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GSDMD Mouse Monoclonal Antibody [A8D7]

Usd: 350

Specification

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Catalog# HA601046

GSDMD Mouse Monoclonal Antibody [A8D7]

Application

  • WB

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

GSDMD Mouse Monoclonal Antibody [A8D7]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human GSDMD aa 101-300/484.

Species Reactivity

Human, Mouse

Validated Applications

WB

Molecular Weight

Predicted band size: 53 kDa

Positive Control

PC-3 cell lysate, NIH/3T3 cell lysate, THP-1 cell lysate.

Conjugation

unconjugated

Clone Number

A8D7

RRID

AB_3073484

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2b

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500

Target

Function

Gasdermin D (GSDMD) is a protein that in humans is encoded by the GSDMD gene on chromosome 8. It belongs to the gasdermin family which is conserved among vertebrates and comprises six members in humans, GSDMA, GSDMB, GSDMC, GSDMD, GSDME (DFNA5) and DFNB59 (Pejvakin). Members of the gasdermin family are expressed in a variety of cell types including epithelial cells and immune cells. GSDMA, GSDMB, GSDMC, GSDMD and GSDME have been suggested to act as tumour suppressors. Several current studies have revealed that GSDMD serves as a specific substrate of inflammatory caspases (caspase-1, -4, -5 and -11) and as an effector molecule for the lytic and highly inflammatory form of programmed cell death known as pyroptosis. Hence, GSDMD is an essential mediator of host defence against microbial infection and danger signals. The pore-forming activity of the N-terminal cleavage product causes cell swelling and lysis to prevent intracellular pathogens from replicating, and is required for the release of cytoplasmic content such as the inflammatory cytokine interleukin-1β (IL-1β) into the extracellular space to recruit and activate immune cells to the site of infection. GSDMD has an additional potential role as an antimicrobial by binding to cardiolipin (CL) and form pores on bacterial membranes. Mutation of GSDMD is associated with various genetic diseases and human cancers, including brain, breast, lung, urinary bladder, cervical, skin, oral cavity, pharynx, colon, liver, cecum, stomach, pancreatic, prostate, oesophageal, head and neck, hematologic, thyroid and uterine cancers.

Background References

1. Shi H. et. al. GSDMD-Mediated Cardiomyocyte Pyroptosis Promotes Myocardial I/R Injury. Circ Res. 2021 Jul

2. Wang K. et. al. Structural Mechanism for GSDMD Targeting by Autoprocessed Caspases in Pyroptosis. Cell. 2020 Mar

Subcellular Location

Cytosol, Inflammasome; Secreted, Cell membrane.

UNIPROT

SwissProt: P57764 Human

SwissProt: Q9D8T2 Mouse

Synonyms

1810036L03Rik antibody

DF 5L antibody

DF5L antibody

DFNA 5L antibody

DFNA5L antibody

FKSG 10 antibody

FKSG10 antibody

FLJ12150 antibody

Gasdermin antibody

Gasdermin D antibody

Expand

Images

  • Western blot analysis of GSDMD on different lysates with Rabbit anti-GSDMD antibody (<a href="/products/HA601046" style="font-weight: bold;text-decoration: underline;">HA601046</a>) at 1/1000 dilution.<br /><br />Lane 1: PC-3M<br /><br />Lysates/proteins at 10 µg/Lane.<br />Exposure time: 120 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA601046" style="font-weight: bold;text-decoration: underline;">HA601046</a>, 1/1000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), RT 2h<br />Secondary antibody: Goat anti-Mouse IgG-HRP (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 52.8 kDa<br />Observed band size: 53 kDa

    Western blot analysis of GSDMD on different lysates with Rabbit anti-GSDMD antibody (HA601046) at 1/1000 dilution.

    Lane 1: PC-3M

    Lysates/proteins at 10 µg/Lane.
    Exposure time: 120 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA601046, 1/1000 in primary antibody dilution buffer (K1803), RT 2h
    Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 52.8 kDa
    Observed band size: 53 kDa

  • Western blot analysis of GSDMD on different lysates with Rabbit anti-GSDMD antibody (<a href="/products/HA601046" style="font-weight: bold;text-decoration: underline;">HA601046</a>) at 1/500 dilution.<br /><br />Lane 1:NIH-3T3<br /><br />Lysates/proteins at 10 µg/Lane.<br />Exposure time: 120 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA601046" style="font-weight: bold;text-decoration: underline;">HA601046</a>, 1/500 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), RT 2h<br />Secondary antibody: Goat anti-Mouse IgG-HRP (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>), 1/100,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 52.8 kDa<br />Observed band size: 53 kDa

    Western blot analysis of GSDMD on different lysates with Rabbit anti-GSDMD antibody (HA601046) at 1/500 dilution.

    Lane 1:NIH-3T3

    Lysates/proteins at 10 µg/Lane.
    Exposure time: 120 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA601046, 1/500 in primary antibody dilution buffer (K1803), RT 2h
    Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/100,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 52.8 kDa
    Observed band size: 53 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

GSDMD Mouse Monoclonal Antibody [A8D8]

Application: WB

Reactivity: Human

Conjugate: unconjugated