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CD161 Mouse Monoclonal Antibody [A8E1]

Usd: 350

Specification

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Catalog# HA601063

CD161 Mouse Monoclonal Antibody [A8E1]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

CD161 Mouse Monoclonal Antibody [A8E1]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within human CD161 aa 176-225.

Species Reactivity

Human

Validated Applications

WB, IHC-P, FC

Molecular Weight

Predicted band size: 25 kDa

Positive Control

THP-1 cell lysate, HL-60 cell lysate, K-562 cell lysate, Jurkat cell lysate, human kidney tissue lysate, human kidney tissue, THP-1.

Conjugation

unconjugated

Clone Number

A8E1

RRID

AB_3071786

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2b

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:500

  • FC

  • 1:500-1:1,000

Target

Function

Killer cell lectin-like receptor subfamily B, member 1, also known as KLRB1, NKR-P1A or CD161 (cluster of differentiation 161), is a human gene. Natural killer (NK) cells are lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein, NKR-P1A or CD161, is classified as a type II membrane protein because it has an external C terminus. NKR-P1A, the receptor encoded by the KLRB1 gene, recognizes Lectin Like Transcript-1 (LLT1) as a functional ligand. Expressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype.

Background References

1. Christiansen D., Mouhtouris E., Milland J., Zingoni A., Santoni A., Sandrin M.S. Recognition of a carbohydrate xenoepitope by human NKRP1A (CD161). Xenotransplantation 13:440-446(2006).

2. Pozo D., Vales-Gomez M., Mavaddat N., Williamson S.C., Chisholm S.E., Reyburn H. CD161 (human NKR-P1A) signaling in NK cells involves the activation of acid sphingomyelinase. J. Immunol. 176:2397-2406(2006).

Subcellular Location

Membrane

UNIPROT

SwissProt: Q12918 Human

Synonyms

C-type lectin domain family 5 member B antibody

CD161 antibody

CLEC5B antibody

HNKR-P1a antibody

Killer Cell Lectin like Receptor Subfamily B Member 1 antibody

Killer cell lectin-like receptor subfamily B member 1 antibody

KLRB1 antibody

KLRB1_HUMAN antibody

Natural killer cell surface protein P1A antibody

NKR antibody

Expand

Images

  • Western blot analysis of CD161 on different lysates with Mouse anti-CD161 antibody (<a href="/products/HA601063" style="font-weight: bold;text-decoration: underline;">HA601063</a>) at 1/2,000 dilution.<br /><br />Lane 1: THP-1 cell lysate (20 µg/Lane)<br />Lane 2: HL-60 cell lysate (20 µg/Lane)<br />Lane 3: K-562 cell lysate (20 µg/Lane)<br />Lane 4: Jurkat cell lysate (20 µg/Lane)<br />Lane 5: Human kidney tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 25 kDa<br />Observed band size: 25 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601063" style="font-weight: bold;text-decoration: underline;">HA601063</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (<a href="/products/NBI02H" style="font-weight: bold;text-decoration: underline;">NBI02H</a>) at 1/5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CD161 on different lysates with Mouse anti-CD161 antibody (HA601063) at 1/2,000 dilution.

    Lane 1: THP-1 cell lysate (20 µg/Lane)
    Lane 2: HL-60 cell lysate (20 µg/Lane)
    Lane 3: K-562 cell lysate (20 µg/Lane)
    Lane 4: Jurkat cell lysate (20 µg/Lane)
    Lane 5: Human kidney tissue lysate (40 µg/Lane)

    Predicted band size: 25 kDa
    Observed band size: 25 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601063) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-CD161 antibody (<a href="/products/HA601063" style="font-weight: bold;text-decoration: underline;">HA601063</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601063" style="font-weight: bold;text-decoration: underline;">HA601063</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-CD161 antibody (HA601063) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601063) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of THP-1 cells labeling CD161.<br /><br />Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (<a href="/products/HA601063" style="font-weight: bold;text-decoration: underline;">HA601063</a>, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Mouse IgG Secondary antibody (<a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of THP-1 cells labeling CD161.

    Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601063, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation