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NAPSIN A Mouse Monoclonal Antibody [A9C4]

Usd: 350

Specification

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Catalog# HA601097

NAPSIN A Mouse Monoclonal Antibody [A9C4]

Application

  • IHC-P

  • IF-Cell

  • IF-Tissue

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

NAPSIN A Mouse Monoclonal Antibody [A9C4]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human NAPSIN A aa 51-200 / 420.

Species Reactivity

Human

Validated Applications

IHC-P, IF-Cell, IF-Tissue

Molecular Weight

Predicted band size: 45 kDa

Positive Control

Human kidney tissue, human lung carcinoma tissue, A549.

Conjugation

unconjugated

Clone Number

A9C4

RRID

AB_3071819

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:4,000

  • IF-Cell

  • 1:200

  • IF-Tissue

  • 1:1,000

Target

Function

Napsin-A is an aspartic proteinase that is encoded in humans by the NAPSA gene. The name napsin comes from novel aspartic proteinase of the pepsin family. The activation peptide of an aspartic proteinase acts as an inhibitor of the active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The pronapsin A gene is expressed predominantly in lung and kidney. Its translation product is predicted to be a fully functional, glycosylated aspartic proteinase precursor containing an RGD motif and an additional 18 residues at its C-terminus. Detection of NAPSA gene expression can be used to distinguish adenocarcinomas from other forms of lung cancer.

Background References

1. Weidemann S et al. Napsin A Expression in Human Tumors and Normal Tissues. Pathol Oncol Res. 2021 Apr

2. Wu J et al. Napsin A Expression in Subtypes of Thyroid Tumors: Comparison with Lung Adenocarcinomas. Endocr Pathol. 2020 Mar

Subcellular Location

Secreted.

UNIPROT

SwissProt: O96009 Human

Synonyms

Asp 4 antibody

ASP4 antibody

Aspartyl protease 4 antibody

KAP antibody

Kdap antibody

Kidney derived aspartic protease like protein antibody

NAP1 antibody

NAPA antibody

Napsa antibody

NAPSA_HUMAN antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-NAPSIN A antibody (<a href="/products/HA601097" style="font-weight: bold;text-decoration: underline;">HA601097</a>) at 1/4,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601097" style="font-weight: bold;text-decoration: underline;">HA601097</a>) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-NAPSIN A antibody (HA601097) at 1/4,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601097) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Mouse anti-NAPSIN A antibody (<a href="/products/HA601097" style="font-weight: bold;text-decoration: underline;">HA601097</a>) at 1/4,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601097" style="font-weight: bold;text-decoration: underline;">HA601097</a>) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Mouse anti-NAPSIN A antibody (HA601097) at 1/4,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601097) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Mouse anti-NAPSIN A antibody (<a href="/products/HA601097" style="font-weight: bold;text-decoration: underline;">HA601097</a>) at 1/4,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601097" style="font-weight: bold;text-decoration: underline;">HA601097</a>) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Mouse anti-NAPSIN A antibody (HA601097) at 1/4,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601097) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human colon tissue (Negative control) with Mouse anti-NAPSIN A antibody (<a href="/products/HA601097" style="font-weight: bold;text-decoration: underline;">HA601097</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601097" style="font-weight: bold;text-decoration: underline;">HA601097</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human colon tissue (Negative control) with Mouse anti-NAPSIN A antibody (HA601097) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601097) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunocytochemistry analysis of A549 cells labeling NAPSIN A with Mouse anti-NAPSIN A antibody (<a href="/products/HA601097" style="font-weight: bold;text-decoration: underline;">HA601097</a>) at 1/200 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-NAPSIN A antibody (<a href="/products/HA601097" style="font-weight: bold;text-decoration: underline;">HA601097</a>) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of A549 cells labeling NAPSIN A with Mouse anti-NAPSIN A antibody (HA601097) at 1/200 dilution.

    Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-NAPSIN A antibody (HA601097) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: lung carcinoma<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/1,000

    Application: IF-Tissue

    Species: Human

    Site: lung carcinoma

    Sample: Paraffin-embedded section

    Antibody concentration: 1/1,000

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