HIV1 p24 Recombinant Mouse Monoclonal Antibody [12G4-R] - BSA and Azide free (Capture)
Overview
Product Name
HIV1 p24 Recombinant Mouse Monoclonal Antibody [12G4-R] - BSA and Azide free (Capture)
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within HIV1 p24 protein aa 133-363 (Catalog#HA210278).
Species Reactivity
HIV1
Validated Applications
ELISA(Cap)
Molecular Weight
Predicted band size: 24 kDa
Positive Control
Recombanint HIV1 p24 protein.
Conjugation
unconjugated
Clone Number
12G4-R
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Cap)
-
1:1,000
Target
Function
One distinctive HIV antigen is a viral protein called p24, a structural protein that makes up most of the HIV viral core, or 'capsid'. There are approximately 2000 molecules per virus particle, or at a molecule weight of 24 kDa, about 104 virus particles per picogram of p24. The onset of symptoms of AIDS correlates with a reduction in the number of CD4+ T-cells and increased levels of virus and p24 in the blood. It is a component of the gag polyprotein. High levels of p24 are present in the blood serum of newly infected individuals during the short period between infection and seroconversion, making p24 antigen assays useful in diagnosing primary HIV infection. Antibodies to p24 are produced during seroconversion, rendering p24 antigen undetectable after seroconversion in most cases. Therefore, p24 antigen assays are not reliable for diagnosing HIV infection after its very earliest stages. Fourth-generation HIV immunoassays detect viral p24 protein in the blood (as well as patient antibodies against the virus). Previous generation tests relied on detecting patient antibodies alone; it takes about 3–4 weeks for the earliest antibodies to be detected. The p24 protein can be detected in patient blood as early as 2 weeks after HIV infection, further reducing the window period necessary to accurately detect the HIV status of the patient.
Background References
1. Chukkapalli V. et al. Evidence in support of RNA-mediated inhibition of phosphatidylserine-dependent HIV-1 Gag membrane binding in cells. J. Virol. 87:7155-7159(2013).
2. Saad J.S. et al. Structural basis for targeting HIV-1 Gag proteins to the plasma membrane for virus assembly. Proc. Natl. Acad. Sci. U.S.A. 103:11364-11369(2006).
Subcellular Location
Virion.
UNIPROT
Synonyms
CA antibody
Capsid protein p24 antibody
HIV-1 Gag p24 antibody
HIV1gp1 antibody
Human immunodeficiency virus 1 antibody
Human immunodeficiency virus type 1 p24 antibody
Images
-
![Standard curve of p24 matched pair antibodies:<br /><br />Sandwich ELISA analysis of p24 matched piar antibodies<br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody <a href="/products/HA601104" style="font-weight: bold;text-decoration: underline;">HA601104</a> [12G4-R] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150ul 1% BSA/PBST blocking buffer, and incubated with serial diluted recombinant p24 protein starting from 1000pg/ml to 15.6pg/ml for 1 hours at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody <a href="/products/HA601105" style="font-weight: bold;text-decoration: underline;">HA601105</a> [12G2-R] (Biotin, 1:2,000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.<br /><br />Capture/detect antibodies, recombinant p24 protein, Streptavidin-HRP are diluted in 1% BSA/PBST.](https://storage.huabio.cn/huabio/productImg/HA601104_1.jpg?v=20260310114346)
Standard curve of p24 matched pair antibodies:
Sandwich ELISA analysis of p24 matched piar antibodies
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA601104 [12G4-R] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150ul 1% BSA/PBST blocking buffer, and incubated with serial diluted recombinant p24 protein starting from 1000pg/ml to 15.6pg/ml for 1 hours at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody HA601105 [12G2-R] (Biotin, 1:2,000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Capture/detect antibodies, recombinant p24 protein, Streptavidin-HRP are diluted in 1% BSA/PBST.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"