CD3 Recombinant Mouse Monoclonal Antibody [PD01-43]
Catalog# HA601110
CD3 Recombinant Mouse Monoclonal Antibody [PD01-43]
-
WB
-
IHC-P
-
IF-Tissue
-
Human
-
unconjugated
Overview
Product Name
CD3 Recombinant Mouse Monoclonal Antibody [PD01-43]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within human CD3E aa 158-207/207.
Species Reactivity
Human
Validated Applications
WB, IHC-P, IF-Tissue
Molecular Weight
Predicted band size: 23 kDa
Positive Control
Jurkat cell lysates, human tonsil tissue, human appendix tissue, human liver tissue.
Conjugation
unconjugated
Clone Number
PD01-43
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
-
1:1,000
-
IF-Tissue
-
1:1,000
Target
Function
The CD3 protein is a T-cell marker, a complex of four structurally distinct membrane glycoprotein isoforms, 20-50 kDa, comprising extracellular, transmembrane and intracellular domains. The CD3 complex is responsible for mediating signal transduction to the internal environment upon antigenic recognition by TCR, causing T-cell proliferation and release of cytokines. Except for a weak expression in Purkinje cells (with some of the Abs) and activated NK-cells, CD3 is found only in T-cells. CD3 appear in the cytoplasm of prothymocytes, and on the surface of about 95% of thymocytes, while cytoplasmic CD3 is lost as the cells differentiate into medullary thymocytes. In therapy resistant celiac disease, a shift from membranous to cytoplasmic CD3 expression is seen (together with loss of CD8). In malignant lymphomas, CD3 is a pan-T-cell lineage-restricted antigen, detected in 80-97% of the T-cell lymphomas. Mature T-cell lymphomas including cases of mycosis fungoides, peripheral T-cell lymphoma and anaplastic large cell lymphoma may aberrantly lose CD3. NK-cell lymphomas can show a cytoplasmic reaction. Reed-Sternberg cells may show a globular paranuclear reaction. CD3 is an important marker in the classification of malignant lymphomas and lymphoid leukaemias. Also the marker is useful for the identification of T-cells in, e.g., celiac disease, lymphocytic colitis and colorectal carcinomas associated with loss of a mismatch repair protein.
Background References
1. Erman B. et. al. Biallelic Form of a Known CD3E Mutation in a Patient with Severe Combined Immunodeficiency. J Clin Immunol. 2020 Apr
2. Chen Q. et. al. CD3(+)CD20(+) T cells and their roles in human diseases. Hum Immunol. 2019 Mar
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
4930549J05Rik antibody
A430104F18Rik antibody
AW552088 antibody
Cd247 antibody
CD247 antigen antibody
CD247 antigen, zeta subunit antibody
CD247 molecule antibody
CD3 antibody
CD3 antigen, delta subunit antibody
CD3 delta antibody
ExpandImages
-
Western blot analysis of CD3 on Jurkat cell lysates with Mouse anti-CD3 antibody (HA601110) at 1/1,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 20 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601110) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD3 antibody (HA601110) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601110) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-CD3 antibody (HA601110) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601110) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-CD3 antibody (HA601110) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601110) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-Tissue
Species: Human
Site: tonsil
Sample: Paraffin-embedded section
Antibody concentration: 1/1,000 -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Appendix
Sample: Paraffin-embedded section
Primary antibody dilution: 1/200
Antigen retrieval: ER2
Platform: Leica Biosystems BOND® RX
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
CD3 Recombinant Rabbit Monoclonal Antibody [PSH19-70]
Application: WB,IHC-P,IF-Cell,FC,mIHC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
CD3 Recombinant Rabbit Monoclonal Antibody [PSH19-70] - BSA and Azide free
Application: WB,IHC-P,IF-Cell,FC,mIHC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
CD3 Recombinant Rabbit Monoclonal Antibody
Application: mIHC
Reactivity: Human
Conjugate: unconjugated
