POMC Recombinant Mouse Monoclonal Antibody [PD01-40]
Catalog# HA601113
POMC Recombinant Mouse Monoclonal Antibody [PD01-40]
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IHC-P
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IF-Tissue
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IHC-Fr
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IF-Cell
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
POMC Recombinant Mouse Monoclonal Antibody [PD01-40]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human POMC aa 1-150 / 267.
Species Reactivity
Human, Mouse, Rat
Validated Applications
IHC-P, IF-Tissue, IHC-Fr, IF-Cell, WB
Target Molecular Weight
Predicted band size: 29 kDa
Positive Control
Mouse pituitary tissue, rat pituitary tissue, mouse hypothalamus (arcuate nucleus) tissue.
Conjugation
unconjugated
Clone Number
PD01-40
RRID
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | IHC-Fr | IF-Tissue | |
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| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IHC-P
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1:10,000-1:20,000
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IF-Tissue
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1:5,000
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IHC-Fr
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1:1,000
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IF-Cell
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1:500
Target
Function
Pro-opiomelanocortin (POMC) is a precursor polypeptide with 241 amino acid residues. POMC is synthesized in corticotrophs of the anterior pituitary from the 267-amino-acid-long polypeptide precursor pre-pro-opiomelanocortin (pre-POMC), by the removal of a 26-amino-acid-long signal peptide sequence during translation. POMC is part of the central melanocortin system. This gene encodes a preproprotein that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the preproprotein and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. The antimicrobial melanotropin alpha peptide exhibits antibacterial and antifungal activity. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described.
Background References
1. Quarta C et al. POMC neuronal heterogeneity in energy balance and beyond: an integrated view. Nat Metab. 2021 Mar
2. Clément K et al. Efficacy and safety of setmelanotide, an MC4R agonist, in individuals with severe obesity due to LEPR or POMC deficiency: single-arm, open-label, multicentre, phase 3 trials. Lancet Diabetes Endocrinol. 2020 Dec
Subcellular Location
Secreted.
Synonyms
ACTH antibody
Adrenocorticotropic hormone antibody
Adrenocorticotropin antibody
alpha melanocyte stimulating hormone antibody
Alpha MSH antibody
Alpha-MSH antibody
beta endorphin antibody
Beta LPH antibody
beta melanocyte stimulating hormone antibody
Beta MSH antibody
ExpandImages
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Application: Immunofluorescence (IHC-Fr)
Species: Rat
Tissue: Pituitary
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA601113, 1/1,000, overnight at 4℃.
Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature. -
Application: Immunofluorescence (IHC-Fr)
Species: Rat
Tissue: Pituitary
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA601113, 1/1,000, overnight at 4℃.
Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue with Mouse anti-POMC antibody (HA601113) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601113) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Mouse anti-POMC antibody (HA601113) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601113) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of paraffin-embedded mouse pituitary tissue labeling POMC with Mouse anti-POMC antibody (HA601113) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601113, green) at 1/5,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded mouse hypothalamus (arcuate nucleus) tissue labeling POMC with Mouse anti-POMC antibody (HA601113) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601113, green) at 1/5,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Application: Immunocytochemistry (IF-cell)
Species: Mouse
Sample: AtT-20 (Mouse pituitary tumor cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA601113, 1/500, overnight at 4℃.
Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature.
Counterstain: Beta tubulin (ET1602-4, Red), 1/500, overnight at 4℃. The nuclear counterstain was DAPI (Blue). -
☑ Relative expression (RE)
Western blot analysis of POMC on different lysates with Mouse anti-POMC antibody (HA601113) at 1/5,000 dilution.
Lane 1: Mouse pituitary tissue lysate
Lane 2: Mouse liver tissue lysate (negative)
Lane 3: Rat pituitary tissue lysate
Lane 4: Rat liver tissue lysate (negative)
Lysates/proteins at 10 µg/Lane.
Exposure time: 20 seconds; ECL: K1801
POMC is not expressed in normal liver tissue (PMID: 6177687).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601113, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 29 kDa
Observed band size: 16-35 kDa -
Immunohistochemical analysis of paraffin-embedded human pituitary tissue with Mouse anti-POMC antibody (HA601113) at 1/20,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601113) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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